SuperScript™ IV 一步法 RT-PCR 系统
SuperScript™ IV 一步法 RT-PCR 系统
Invitrogen™

SuperScript™ IV 一步法 RT-PCR 系统

Invitrogen SuperScript IV 一步法 RT-PCR 系统兼具 SuperScript IV 反转录酶的高持续合成能力和 Platinum了解更多信息
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货号包括反应次数
12595100Kit with ezDNase100 次反应
12594100Kit only100 次反应
货号 12595100
价格(CNY)
14,824.00
Each
添加至购物车
包括:
Kit with ezDNase
反应次数:
100 次反应
请求批量或定制报价
价格(CNY)
14,824.00
Each
添加至购物车
Invitrogen SuperScript IV 一步法 RT-PCR 系统兼具 SuperScript IV 反转录酶的高持续合成能力和 Platinum SuperFi DNA 聚合酶的高保真度,可提供无可比拟的一步法 RT-PCR 性能。使用该便利的一步法配方加基因特异性引物,您可在单管中从总 RNA 或 mRNA 中进行 cDNA 合成和 PCR 扩增。

SuperScript IV 一步法 RT-PCR 系统的特点包括:
•具有出色的性能(灵敏度低至 0.01 pg RNA,靶标序列长度可达 13.8 kb)以及更快的一步法 RT-PCR 方案
• 即便是对于纯度不佳的 RNA 样品,也可实现可靠的靶标检测
• 两相热启动活化机制可实现高特异性、高得率和室温配制反应体系

SuperScript IV RT 是一种专有的 MMLV RT 突变体,具有更高的持续合成能力、热稳定性和抑制剂耐受性以及更低的 RNase H 活性,可用于灵敏且高效的全长 cDNA 合成。Platinum SuperFi DNA 聚合酶是一种校正性 DNA 聚合酶,兼具出色的保真度与可靠的 Platinum 热启动技术,可较大限度地提高 PCR 成功率。与其他一步法 RT-PCR 试剂盒相比,这两种工程改造酶的组合提高了 RT-PCR 得率和保真度,并且可以扩增更长的模板。

为了获得更佳性能,我们提供含有 ezDNase 的 SuperScript IV 一步法 RT-PCR 系统。ezDNase 通过极其简化的基因组 DNA 去除步骤进一步增强了一步法 RT-PCR 工作流程,可确保了一步法 RT-PCR 结果的极高准确度和置信度。

仅供科研使用。不可用于诊断程序。
规格
最终产品类型PCR 扩增 cDNA
产品规格试剂盒
热启动内置热启动
包括Kit with ezDNase
反应次数100 次反应
最佳反应温度45°C 至 60°C
聚合酶Platinum SuperFi
数量Each
反应形式预混液
试剂类型反转录
逆转录酶SuperScript IV
核糖核酸酶 H 活性减少
运输条件干冰
尺寸(最终产品)长达 14 kb
原始材料RNA
技术1 步法 RT-PCR
检测方法引物-探针
高 GC PCR 扩增效果
PCR 方法一步法 RT-qPCR
反应速度10 min。
Unit SizeEach
内容与储存

• SuperScript IV RT 混合物 (50 μL)
• 2X Platinum SuperFi RT-PCR 预混液 (2 x 1.25 mL)
• ezDNase 酶 (100 μL)
• 10X ezDNase 缓冲液 (100 μL)
• 无核酸酶水 (2 x 1.25 mL)

储存在 –20°C 下。

常见问题解答 (FAQ)

In your SuperScript IV RT protocols, there is no ezDNase inactivation step. Will active ezDNase affect RNA or the RT reaction?

The Invitrogen ezDNase Enzyme is a novel DNase that is highly specific for double-stranded DNA. It has no activity on single-stranded DNA in RT reactions (primers or probes), or on RNA. The enzyme is also thermolabile—it is inactivated quickly at temperatures typical for the SuperScript IV RT reaction (e.g., 50°C). The additional inactivation step is therefore not required in RT-qPCR applications.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Which SuperScript IV RT format do you recommend for real-time PCR applications?

For RT-qPCR applications we recommend using the Invitrogen SuperScript IV VILO Master Mix (Cat. No. 11756050). The cDNA synthesis reaction setup with this master mix requires fewer pipetting steps and therefore reduces variation in the data. SuperScript IV RT, as a component of the master mix, offers the highest efficiency of cDNA synthesis step compared to competitors’ products.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Are there any significant changes in the SuperScript IV RT protocol compared to the SuperScript III RT protocol?

The only change is that the incubation time for the reverse transcription reaction has been reduced from 50 minutes to 10 minutes. All the other parameters and steps are the same.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Can I get comparable cDNA yield and length using the SuperScript IV RT 10-minute protocol as when using the 50-minute protocol for SuperScript III RT?

When compared with SuperScript III RT (and other manufacturers’ RTs) in a synthesis reaction for a 9 kb cDNA, SuperScript IV RT performed successful synthesis in just 10 minutes and did so with comparable (or improved) yield (as shown by gel band density).

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Do you offer a one-step RT-qPCR kit containing SuperScript IV RT?

We have not created a one-step RT-qPCR kit containing SuperScript IV RT for real-time PCR. However, it is included in end-point one-step RT-PCR kits: https://thermofisher.com/ssiv-onestep

We also offer a two-step RT-qPCR kit containing SuperScript IV RT: https://www.thermofisher.com/vilo

For one-step RT-qPCR, we offer a kit with SuperScript III RT: https://www.thermofisher.com/order/catalog/product/117320200