A comparison between different human hepatocyte models reveals profound differences in net glucose production, lipid composition and metabolism in vitro.
AuthorsBonanini F,Singh M,Yang H,Kurek D,Harms AC,Mardinoglu A,Hankemeier T
JournalExperimental cell research
PubMed ID38499143
A simple tool to improve pluripotent stem cell differentiation.
AuthorsChetty S, Pagliuca FW, Honore C, Kweudjeu A, Rezania A, Melton DA,
JournalNat Methods
PubMed ID23584186
We describe a method to help overcome restrictions on the differentiation propensities of human pluripotent stem cells. Culturing pluripotent stem cells in dimethylsulfoxide (DMSO) activates the retinoblastoma protein, increases the proportion of cells in the early G1 phase of the cell cycle and, in more than 25 embryonic and induced ... More
A rapid, cost-effective method for counting human embryonic stem cell numbers as clumps.
AuthorsProwse A, Wolvetang E, Gray P,
JournalBiotechniques
PubMed ID19594444
Enumeration of human embryonic stem cell (hESC) numbers through single cell digestion can be time consuming especially in high-throughput or multi-factorial analysis containing 50+ samples. We have developed a reproducible, cost-effective method of counting hESCs in clumps circumventing the need to manually dissociate each sample to single cells. The method ... More