A simple tool to improve pluripotent stem cell differentiation.
AuthorsChetty S, Pagliuca FW, Honore C, Kweudjeu A, Rezania A, Melton DA,
JournalNat Methods
PubMed ID23584186
We describe a method to help overcome restrictions on the differentiation propensities of human pluripotent stem cells. Culturing pluripotent stem cells in dimethylsulfoxide (DMSO) activates the retinoblastoma protein, increases the proportion of cells in the early G1 phase of the cell cycle and, in more than 25 embryonic and induced ... More