Search
Gibco™
Advanced RPMI 1640 Medium
Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cellsRead more
Have Questions?
Change view
| Catalog Number | Quantity |
|---|---|
| 12633020 | 10 x 500 mL |
| 12633012 | 500 mL |
Catalog number 12633020
Price (CNY)
5,717.00
Each
Quantity:
10 x 500 mL
Price (CNY)
5,717.00
Each
Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic RPMI 1640, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.i Cells successfully cultured in Advanced RPMI 1640, with no adaptation, include Sp2, Vero, Raji, Daudi, and Jurkat.
This Advanced RPMI 1640 is manufactured as follows:
With: glucose, non-essential amino acids, sodium pyruvate, phenol red
Without: L-glutamine, HEPES
The complete formulation is available.
Gibco Advanced RPMI 1640 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.
Advanced RPMI 1640 requires supplementation with 1-5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX supplement. Many cell lines do not require adaptation to this medium. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced RPMI 1640 uses a sodium bicarbonate buffer system (2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
iAdvanced Media: Reduced Sera Solutions. Quest, 2004. 1.3:26-30.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell LineSp2, Vero, Raji, Daudi, and Jurkat
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Product TypeRPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Quantity10 x 500 mL
Shelf Life12 Months From Date of Manufacture
ClassificationAnimal Origin
FormLiquid
Serum LevelReduced Serum
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesGlucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
Without AdditivesNo Glutamine, No HEPES
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture