Sf-900™ III SFM
Invitrogen17万+抗体限时买二赠一,靶点广,灵活用!
Invitrogen17万+抗体限时买二赠一,靶点广,灵活用!
Sf-900™ III SFM
Actual product may vary
Sf-900™ III SFM
Sf-900™ III SFM
Gibco™

Sf-900™ III SFM

Sf-900™ III SFM 是一种低水解物、无血清、无蛋白、非动物源性–昆虫细胞培养基,其经过优化,可用于草地贪夜蛾(Sf9 和 Sf21了解更多信息
Have Questions?
更改视图buttonViewtableView
货号数量
12658019500 mL
126580271000 mL
1265803510 L
1265800120 L
货号 12658019
价格(CNY)
937.00
Each
添加至购物车
数量:
500 mL
Customize this product
价格(CNY)
937.00
Each
添加至购物车
Sf-900™ III SFM 是一种低水解物、无血清、无蛋白、非动物源性–昆虫细胞培养基,其经过优化,可用于草地贪夜蛾(Sf9 和 Sf21)细胞的生长和维持,并可利用杆状病毒和稳定昆虫表达系统进行重组基因表达。该培养基适用于悬浮和单层培养方法,并支持其他鳞翅目昆虫细胞系的生长。Gibco™ Sf-900™ III SFM 的特点:

•优异的长期、高密度生长
•经优化用于重组蛋白生产
•无血清、无蛋白、无动物来源–、即用型制剂
•改善的批间一致性

优异的长期、高密度生长
草地贪夜蛾 (Sf9) 细胞在 Sf-900™ III 中生长达到最大细胞密度 10 至 14 × 106 个细胞/mL,与 Sf-900™ II SFM 和其他市售无血清培养基相比显著改善(参见图)。

优化用于重组蛋白生产
传统情况下,补充 10% FBS 的 Grace 培养基已用于重组蛋白表达。Sf-900™ III SFM 是一种改善的无血清、无蛋白、非动物源性–培养基,设计用于培养 Sf9 和其他鳞翅目昆虫细胞系以及生产昆虫病毒和重组蛋白。

无血清、无蛋白、非动物源性–、即用型配方
Gibco™ Sf-900™ III SFM 是一种无血清、无蛋白、非动物源性–培养基,可更容易地纯化您的目标蛋白质。Sf-900™ III SFM 为即用型;不需要添加血清、谷氨酰胺或表面活性剂。应将适用于其他市售无血清培养基的细胞依次调整至 Sf-900™ II SFM(有关详细信息参见产品手册)。

改善的批间一致性
Gibco™ Sf-900™ III SFM 含有降低的水解物浓度,与 Sf-900™ III SFM 相比,改善了批间一致性(参见图)。

cGMP 生产和质量体系
Gibco™ Sf-900 II SFM 在位于纽约格兰德岛的符合 cGMP 要求的工厂内生产。该工厂是在 FDA 注册的医疗器械生产商,通过 ISO 13485 标准认证。
仅供科研使用。不可用于诊断程序。
规格
细胞系Sf21, Sf9
细胞类型昆虫细胞
产品线Gibco, Sf-900
产品类型昆虫细胞无血清培养基 (SFM)
数量500 mL
运输条件室温
种属草地贪夜蛾 (S. frugiperda, Spodoptera frugiperda)
分类非动物源性, 无蛋白, 无血清
形式液体
血清水平无血清
加有添加剂谷氨酰胺
Unit SizeEach
内容与储存
储存条件:2-8°C。避光储存
运输条件:环境条件
有效期:自生产之日起 18 个月

常见问题解答 (FAQ)

昆虫细胞培养可使用哪些种类和浓度的杀菌剂?

许多抗生素都适用于昆虫细胞。下列为常用抗生素:

青霉素/链霉素:50–100 U/mL;50–100 μg/mL
两性霉素B(Fungizone 抗真菌剂):0.25 μg/mL
庆大霉素:500 mL培养基中加入0.5 mL 10 mg/mL溶液(最终浓度10 μg/mL)

在将血清加入我的培养基之前,是否需要对其进行热灭活操作?

热灭活操作不是必需的。我们的团队直接使用未经热灭活的血清,并未发现其对细胞生长或细胞形态有任何显著影响。

你们是否提供无血清的昆虫培养基?

是的,我们提供各类无血清的昆虫培养基,请点击此处(https://www.thermofisher.com/us/en/home/life-science/cell-culture/insect-cell-culture/insect-cell-culture-misc/serum-free-media.html?icid=cvc-insect-media-c2t2)浏览我们所提供的培养基以及它们之间的区别。

What antimicrobials can be used in insect culture and at what concentration?

Many antibiotics are suitable for use with insect cells. The following antibiotics are commonly used:
- Penicillin/Streptomycine: 50-100 U/mL; 50-100 µg/mL
- Amphotericin B (Fungizone antimycotic): 0.25 µg/mL
- Gentamicin: 0.5 mL of 10 mg/mL solution in 500 mL media (final concentration: 10 µg/mL)

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is it necessary to heat-inactivate my serum before adding it to my medium?

Heat inactivation is not necessary. Our team has routinely used serum that has not been heat-inactivated, and we have not observed any effect on cell growth or morphology.

Many cells do not require heat-inactivated FBS. Some cells prefer heat-inactivated FBS. For instance, we use heat-inactivated FBS for our insect cell lines, i.e., Sf9 and Sf21 cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

引用和文献 (2)

引用和文献
Abstract
Predictors of seropositivity to human papillomavirus type 53: one of the most prevalent high risk-related cervical human papillomaviruses.
Authors:Malik ZA, Hailpern SM, Burk RD,
Journal:Viral Immunol
PubMed ID:18681800
'Persistent cervicovaginal infection with high-risk types of HPV is the major risk factor for subsequent cervical neoplasia. HPV53, part of the alpha 6 species group along with HPV types 30, 56, and 66, is one of the most prevalent high risk-related HPV types, yet little is known about the molecular ... More
The S100A8-serum amyloid A3-TLR4 paracrine cascade establishes a pre-metastatic phase.
Authors:Hiratsuka S, Watanabe A, Sakurai Y, Akashi-Takamura S, Ishibashi S, Miyake K, Shibuya M, Akira S, Aburatani H, Maru Y,
Journal:Nat Cell Biol
PubMed ID:18820689
A large number of macrophages and haematopoietic progenitor cells accumulate in pre-metastatic lungs in which chemoattractants, such as S100A8 and S100A9, are produced by distant primary tumours serving as metastatic soil. The exact mechanism by which these chemoattractants elicit cell accumulation is not known. Here, we show that serum amyloid ... More