Sf-900™ III SFM 是一种低水解物、无血清、无蛋白、非动物源性–昆虫细胞培养基,其经过优化,可用于草地贪夜蛾(Sf9 和 Sf21了解更多信息
Have Questions?
更改视图
货号
数量
12658019
500 mL
12658027
1000 mL
12658035
10 L
12658001
20 L
4 选项
货号 12658019
价格(CNY)
937.00
Each
添加至购物车
数量:
500 mL
Customize this product
价格(CNY)
937.00
Each
添加至购物车
Sf-900™ III SFM 是一种低水解物、无血清、无蛋白、非动物源性–昆虫细胞培养基,其经过优化,可用于草地贪夜蛾(Sf9 和 Sf21)细胞的生长和维持,并可利用杆状病毒和稳定昆虫表达系统进行重组基因表达。该培养基适用于悬浮和单层培养方法,并支持其他鳞翅目昆虫细胞系的生长。Gibco™ Sf-900™ III SFM 的特点:
无血清、无蛋白、非动物源性–、即用型配方 Gibco™ Sf-900™ III SFM 是一种无血清、无蛋白、非动物源性–培养基,可更容易地纯化您的目标蛋白质。Sf-900™ III SFM 为即用型;不需要添加血清、谷氨酰胺或表面活性剂。应将适用于其他市售无血清培养基的细胞依次调整至 Sf-900™ II SFM(有关详细信息参见产品手册)。
改善的批间一致性 Gibco™ Sf-900™ III SFM 含有降低的水解物浓度,与 Sf-900™ III SFM 相比,改善了批间一致性(参见图)。
cGMP 生产和质量体系 Gibco™ Sf-900 II SFM 在位于纽约格兰德岛的符合 cGMP 要求的工厂内生产。该工厂是在 FDA 注册的医疗器械生产商,通过 ISO 13485 标准认证。
What antimicrobials can be used in insect culture and at what concentration?
Many antibiotics are suitable for use with insect cells. The following antibiotics are commonly used:
- Penicillin/Streptomycine: 50-100 U/mL; 50-100 µg/mL
- Amphotericin B (Fungizone antimycotic): 0.25 µg/mL
- Gentamicin: 0.5 mL of 10 mg/mL solution in 500 mL media (final concentration: 10 µg/mL)
Is it necessary to heat-inactivate my serum before adding it to my medium?
Heat inactivation is not necessary. Our team has routinely used serum that has not been heat-inactivated, and we have not observed any effect on cell growth or morphology.
Many cells do not require heat-inactivated FBS. Some cells prefer heat-inactivated FBS. For instance, we use heat-inactivated FBS for our insect cell lines, i.e., Sf9 and Sf21 cells.
Predictors of seropositivity to human papillomavirus type 53: one of the most prevalent high risk-related cervical human papillomaviruses.
Authors:Malik ZA, Hailpern SM, Burk RD,
Journal:Viral Immunol
PubMed ID:18681800
'Persistent cervicovaginal infection with high-risk types of HPV is the major risk factor for subsequent cervical neoplasia. HPV53, part of the alpha 6 species group along with HPV types 30, 56, and 66, is one of the most prevalent high risk-related HPV types, yet little is known about the molecular ... More
The S100A8-serum amyloid A3-TLR4 paracrine cascade establishes a pre-metastatic phase.
Authors:Hiratsuka S, Watanabe A, Sakurai Y, Akashi-Takamura S, Ishibashi S, Miyake K, Shibuya M, Akira S, Aburatani H, Maru Y,
Journal:Nat Cell Biol
PubMed ID:18820689
A large number of macrophages and haematopoietic progenitor cells accumulate in pre-metastatic lungs in which chemoattractants, such as S100A8 and S100A9, are produced by distant primary tumours serving as metastatic soil. The exact mechanism by which these chemoattractants elicit cell accumulation is not known. Here, we show that serum amyloid ... More