Sf-900™ III SFM
Invitrogen17万+抗体限时买二赠一,靶点广,灵活用!
Invitrogen17万+抗体限时买二赠一,靶点广,灵活用!
Sf-900™ III SFM
Actual product may vary
Sf-900™ III SFM
Sf-900™ III SFM
Gibco™

Sf-900™ III SFM

A low-hydrolysate, serum-free, protein-free, animal origin–free insect cell culture medium
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Catalog NumberQuantity
1265803510 L
12658019500 mL
126580271000 mL
1265800120 L
Catalog number 12658035
Price (CNY)
16,999.00
Each
Add to cart
Quantity:
10 L
Customize this product
Price (CNY)
16,999.00
Each
Add to cart

Sf-900 III SFM is a low-hydrolysate, serum-free, protein-free, animal origin–free insect cell culture medium optimized for the growth and maintenance of Spodoptera frugiperda (Sf9 and Sf21) cells.

  • Optimized for recombinant gene expression using the baculovirus and stable insect expression systems
  • Suitable for suspension and monolayer culture methods and supports growth of other lepidopteran cell lines
  • Spodoptera frugiperda (Sf9) cells grown in Sf-900 III SFM achieve maximum cell densities of 10–14 x 106 cells/mL
  • An improved serum-free, protein-free, animal origin–free medium designed for growth of Sf9 and other lepidopteran cell lines and production of insect viruses and recombinant proteins
  • Ready to use format does not require addition of serum, glutamine, or surfactants
  • Cells adapted to other commercially available serum-free media should be sequentially adapted into Sf-900 III SFM
  • Reduced hydrolysate concentrations improve lot-to-lot consistency

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Specifications
Cell LineSf21, Sf9
Cell TypeInsect Cell
Product LineGibco™, Sf-900™
Product TypeInsect Cell Serum Free Medium (SFM)
Quantity10 L
Shipping ConditionRoom Temperature
SpeciesS. frugiperda, Spodoptera frugiperda
ClassificationAnimal Origin-free, Protein-free, Serum-free
FormLiquid
Serum LevelSerum-free
With AdditivesGlutamine
Unit SizeEach
Contents & Storage
Storage conditions: 2–8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 18 months from date of manufacture

Frequently asked questions (FAQs)

What antimicrobials can be used in insect culture and at what concentration?

Many antibiotics are suitable for use with insect cells. The following antibiotics are commonly used:
- Penicillin/Streptomycine: 50-100 U/mL; 50-100 µg/mL
- Amphotericin B (Fungizone antimycotic): 0.25 µg/mL
- Gentamicin: 0.5 mL of 10 mg/mL solution in 500 mL media (final concentration: 10 µg/mL)

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is it necessary to heat-inactivate my serum before adding it to my medium?

Heat inactivation is not necessary. Our team has routinely used serum that has not been heat-inactivated, and we have not observed any effect on cell growth or morphology.

Many cells do not require heat-inactivated FBS. Some cells prefer heat-inactivated FBS. For instance, we use heat-inactivated FBS for our insect cell lines, i.e., Sf9 and Sf21 cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer serum-free insect media?

Yes, we offer a variety of serum-free insect media. Please go here (http://www.thermofisher.com/us/en/home/life-science/cell-culture/insect-cell-culture/insect-cell-culture-misc/serum-free-media.html?icid=cvc-insect-media-c2t2) to view the media we offer and the differences between them.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will your company continue to support other films?

Yes. Although we feel that the PE film exceeds many of the technical properties of the other available films, we will continue to offer and support our other currently available films through custom orders.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

In what size package will my Gibco Cell Culture Bags ship?

Each bag size will arrive in a specific heavyweight corrugated box designed for shipment:
5 L: 12” x 14.25” x 5.5”
10 L: 13” x 23.5” x 6.125”
20 L: 15” x 24” x 6.125”

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.