AlgiMatrix™ 96 孔,3D 培养系统,平底微孔板
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Gibco™

AlgiMatrix™ 96 孔,3D 培养系统,平底微孔板

AlgiMatrix™ 3D Culture System is the first user-friendly non-animal-derived bioscaffold for three-dimensional (3D) cell culture. AlgiMatrix™ 3D Culture System creates了解更多信息
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货号数量孔数量
126840315 支/包96
126840151 支/包96
货号 12684031
价格(CNY)
25,848.00
Each
添加至购物车
数量:
5 支/包
孔数量:
96
价格(CNY)
25,848.00
Each
添加至购物车
AlgiMatrix™ 3D Culture System is the first user-friendly non-animal-derived bioscaffold for three-dimensional (3D) cell culture. AlgiMatrix™ 3D Culture System creates a cell culture model system that more closely reflects normal cell morphology and behavior, to meet researchers' requirements for 3D cell culture in fields such as toxicology, drug development, cancer and stem cell research, development and morphogenesis, tissue and organ engineering, heart disease, diabetes, and Alzheimers disease.

AlgiMatrix™ 3D Culture System is supplied in a 96-well format that fits well into the workflow of both lower- and higher-throughput users. Scientists can inoculate cells directly into the sterile microtiter plates preloaded with lyophilized alginate sponge, with no pretreatments or preparations required. The combination of convenience, high capacity, and microscopic visualization makes AlgiMatrix™ 3D Culture System an ideal solution for broad basic research and drug discovery applications.

Applications:
AlgiMatrix™ 3D Culture System is suitable for many cell-based screening, drug discovery, and human cell therapy procedures, including the Multicellular Tumor Spheroid Assays (MCTS),1 hepatocyte 2-4 and cardiomyocyte organogenesis studies,5 co-culture studies,6 high-throughput drug screening assays,1 and embryonic stem cell 3D differentiation.7 Each lot is performance and quality tested in a cell-based toxicity assay.

Storage:
Room temperature.

References:
1. Kunz-Schughart, L., et al., (2004) J Biomolec Screening 9, 273.
2. Dvir-Ginzberg, M., et al. (2003) Tissue Engineering 9, 757.
3. Elkayam, T., et al. (2006) Tissue Engineering 12, 1357.
4. Kedem, A., et al. (2005) Tissue Engineering 11, 715.
5. Dar, A., et al. (2002) Biotechnology & Bioengineering 80, 305.
6. Kim, J. (2005) Seminars in Cancer Biology 15, 365.
7. Gerecht-Nir, S., et al. (2004) Biotechnology & Bioengineering 88, 313.
仅供科研使用。不可用于诊断程序。
规格
带盖
每架数量5 支/包
包装5 支/包
产品类型塑料、无涂层、非无菌孔板
纯度或质量等级样品处理:
数量5 支/包
运输条件室温
颜色清除
孔数量96
产品线Algimatrix
无菌无菌
表面处理AlgiMatrix 3D 培养系统
孔形
Unit SizeEach
内容与储存
在室温下储存。

常见问题解答 (FAQ)

AlgiMatrix 基质可以在室温下稳定放置多久而不发生降解?

AlgiMatrix 基质在不开封的条件下可稳定放置12个月。一旦开封后,整板都需及时用掉。

为何Gibco Geltrex 基质适用于粘附相关的研究,而Gibco AlgiMatrix 3D培养系统不适用?

Gibco Geltrex 基质是含有细胞粘附所需的胶原IV,层粘连蛋白及其他基质蛋白和生长因子的基底膜抽提物。AlgiMatrix 基质只含有不具细胞粘附促进能力的藻酸盐。藻酸盐海绵体中没有能与细胞相互作用的成份。

其他的细胞活性检测试剂能与AlgiMatrix 基质一起使用吗?

除了Invitrogen alamarBlue 细胞活力检测试剂(Cell Viability Reagent)之外,AlgiMatrix 基质还能够与Invitrogen PrestoBlue 细胞活力检测试剂,Promega  Cell Titer-Glo 试剂或Invitrogen CyQuant  Direct Cell Proliferation Assay试剂盒相兼容。具体实验方案与2D细胞培养系统几乎相同,但需要加入纯AlgiMatrix 基质作为空白对照组。

钙在固化和解离缓冲液中起着怎样的重要作用?

固化缓冲液通过向海绵体系中加入钙成份来发挥作用——加入后使海绵体变硬。解离缓冲液通过去除海绵体系中的钙成份来发挥作用——加入后使海绵体变软。

使用AlgiMatrix 基质开展分析时,会有多大的变异度?

使用AlgiMatrix 基质的3D细胞培养体系为细胞的生长和行为提供了一个更接近生理的条件,但由于该系统更为复杂,通常也的确会带来更高的结果变异度。您可能需要在实验中设置更多的重复组和对照组。