关于 Platinum Taq DNA 聚合酶 Platinum Taq DNA 聚合酶是一种重组 Taq DNA 聚合酶,其与专有抗体结合,可在室温下抑制酶活性,从而减少在较低温度下发生的非特异性靶标扩增。在初始 PCR 变性步骤中,抗体解离并使 DNA 聚合酶完全恢复其活性。与 Taq DNA 聚合酶一样,Platinum Taq DNA 聚合酶具有非模板依赖性末端转移酶活性,可在产物末端添加 3’ 脱氧腺苷,并且具有 5'→3’ 核酸外切酶活性。
注:为了获得出色的 PCR 性能,我们推荐使用下一代酶 Platinum II Taq 热启动 DNA 聚合酶。Platinum II Taq 热启动 DNA 聚合酶是专为通用引物退火和快速、简易的 PCR 而设计的,其以独特的方式结合了创新的缓冲液、高性能设计的 Taq DNA 聚合酶和更优的热启动技术。Platinum II 热启动 PCR 预混液 (2X) 包含 Platinum II Taq 热启动 DNA 聚合酶,该聚合酶是一种含 Platinum II PCR 缓冲液和 dNTP 的即用型混合物,从而可减少 PCR 反应构建过程中的移液步骤。
仅供科研使用。不可用于诊断程序。
规格
颜色Green
保真度(相对于 Taq)1 X
产品规格管装
热启动内置热启动
反应次数50 次反应
突出端3'-A
聚合酶Platinum Taq DNA 聚合酶
产品类型热启动 PCR 预混液
数量50 reactions
反应形式SuperMix 或预混液
运输条件干冰
尺寸(最终产品)5 kb或更小
原始材料DNA
最大浓度2X
适用于(应用)Hot-start PCR
高 GC PCR 扩增效果中
标签或染料绿色
反应速度标准
Unit SizeEach
内容与储存
每盒都包含: •1.25 mL Platinum Green 热启动 PCR 2X 预混液 •1.25 mL Platinum GC 增强剂 •1.25 mL无核酸酶水
储存在-20°C 的非无霜冰箱中。
常见问题解答 (FAQ)
Do you have any recommendations for troubleshooting the amplification of GC-rich or problematic targets with Platinum Hot Start PCR Master Mix (2X) (Cat. Nos. 13000012, 13000013, 13000014)?
We have the following recommendations:
Increase the extension time from 1 min/kb to 1.5 min/kb.
Vary total PCR cycles from 20-40; 35 cycles is typical.
Try denaturing at 95 degrees C for 45 seconds.
GC-rich or problematic targets work better with MgSO4 instead of MgCl2.
For GC-rich templates, test higher annealing temperatures starting with the temperature that is equal to your primer Tm and in increments of 2 degrees C for up to six different temperatures.
Note: Addition of Platinum GC Enhancer solution enhances the amplification of GC-rich and problematic sequences.
What is the final concentration of MgCl2 in the Platinum Hot Start PCR Master Mix (2X) (Cat. Nos. 13000012, 13000013, 13000014)?
The final concentration is 1.5 mM MgCl2.
When should the GC enhancer be used that comes with the Platinum Green Hot Start PCR 2X Master Mix (2X)?
We recommend using the GC enhancer for targets with >65% GC sequences. We recommend that you have a final concentration of 20% of the GC enhancer in the reaction mix. There is enough GC enhancer included in the kit to add it to every PCR reaction.
Is the green dye that comes with the Platinum Taq Hot Start Green DNA Polymerase and Platinum Green Hot Start PCR Master Mix (2X) compatible with downstream applications?
Yes the green dye is compatible with downstream applications such as fluorescent automatic DNA sequencing, ligation, and restriction digestion.
Do you offer Platinum Taq DNA Polymerase formats that already contain loading dyes in the reaction buffer?
Yes, we offer several Platinum Taq DNA polymerases and their master mixes, supplemented with tracking dyes for direct loading of PCR products on gels:
- Platinum II Hot-Start Green PCR Master Mix (2X)
- Platinum SuperFi Green DNA Polymerase and Platinum SuperFi Green PCR Master Mix
- Platinum Taq Green Hot-Start DNA Polymerase and Platinum Green Hot-Start PCR Master Mix (2X)
- DreamTaq Green Hot-Start DNA Polymerase and DreamTaq Hot-Start Green PCR Master Mix
- Phusion Green Hot-Start II High-Fidelity DNA Polymerase and Phusion Green Hot-Start II High-Fidelity PCR Master Mix.