Invitrogen

NEFH Monoclonal Antibody (RMdO-20)

Name: NEFH Monoclonal Antibody (RMdO-20)
Brand: Invitrogen
SKU: 13-1300
Availability: InStock

高级验证

2个已发表图片

15 篇参考文献

查看其他44个NF-H抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

NEFH Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 15

NEFH Antibody (13-1300) in IHC (P)

Immunohistochemistry analysis of the neurofilament heavy chain showing staining in the filaments of paraffin-embedded mouse brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0) and microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 m... View More

NEFH Antibody in Immunohistochemistry (Frozen) (IHC (F))

NEFH Antibody in Immunocytochemistry (ICC/IF)

NEFH Antibody in Immunohistochemistry (IHC)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

NEFH Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of NEFH was observed in SH-SY5Y and IMR-32 in comparison to LNCaP and MCF7 using Anti-NEFH Monoclonal Antibody (RMdO-20) (Product # 13-1300) in Western Blot. {RE}

Immunogen image

Immunogen Image NEFH Monoclonal Antibody (RMdO-20)

产品信息

13-1300

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5-2 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

查看7篇已发表文章 7篇已发表文章

免疫组化（石蜡） (IHC (P))

1:20

查看2篇已发表文章 2篇已发表文章

免疫组化（冰冻） (IHC (F))

1:20

免疫细胞化学 (ICC/IF)

1:20

查看5篇已发表文章 5篇已发表文章

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

免疫沉淀 (IP)

2-5 µg

产品规格

种属反应

Bovine, Hamster, Human, Mollusc, Mouse, Rabbit, Rat

已发表种属

Human, Mouse, Rabbit, Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

RMdO-20

抗原

Adult Rat neurofilaments

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

200 µg

纯化类型

purified

保存液

PBS

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2532999

产品详细信息

This antibody reacts with 200 kDa subunit protein of human neurofilament. It specifically recognizes a nonphosphorylated epitope in the tail domain of NF-H. Dephosphorylation of NF-H will increase immunoreactivity with this antibody.

This antibody is suitable for immunohistochemical staining of Bouin's and alcohol-fixed paraffin-embedded or frozen tissue sections. To stain, incubate 30-60 minutes at room temperature or overnight at 4°C.

靶标信息

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-H has an important role in mature axons and is involved in amyotrophic lateral sclerosis (ALS).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: 200 kDa neurofilament protein; NEF3; neurofilament; neurofilament 200kDa; Neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide; neurofilament-H; NF 200 kD; NF-H; NHC

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基因别名: I79_012512; Kiaa0845; mKIAA0845; Nefh; NF-H; NF200; Nfh

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UniProt ID: (Mouse) P19246