细胞解离缓冲液，无酶，PBS
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细胞解离缓冲液，无酶，PBS
引用和文献 (3)
Gibco™

细胞解离缓冲液，无酶，PBS

Gibco™ 细胞解离缓冲液是一种膜过滤的等渗无酶盐溶液螯合剂，以及在无钙和无镁的磷酸盐缓冲液 (PBS) 中作为细胞调节剂。Gibco™ 细胞解离缓冲液适用于对哺乳动物细胞进行温和的解离，以进行需要完整细胞表面蛋白的研究，如配体结合、流式细胞术和免疫组织化学。Gibco™ 细胞解离缓冲液适用于轻微贴壁细胞系（例如了解更多信息
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货号数量
13151014100 mL
货号 13151014
价格（CNY)
800.00
Each
添加至购物车
数量:
100 mL
价格（CNY)
800.00
Each
添加至购物车
Gibco™ 细胞解离缓冲液是一种膜过滤的等渗无酶盐溶液螯合剂，以及在无钙和无镁的磷酸盐缓冲液 (PBS) 中作为细胞调节剂。Gibco™ 细胞解离缓冲液适用于对哺乳动物细胞进行温和的解离，以进行需要完整细胞表面蛋白的研究，如配体结合、流式细胞术和免疫组织化学。Gibco™ 细胞解离缓冲液适用于轻微贴壁细胞系（例如 HeLa 和 NIH 3T3），但不建议用于强贴壁细胞的常规传代。解离方案见我们的技术参考库。
仅供科研使用。不可用于诊断程序。
规格
细胞系HeLa, NIH 3T3
螯合剂EDTA
数量100 mL
备注室温稳定
有效期24 个月
运输条件室温
分类无动物来源
产品类型细胞培养解离试剂
无菌无菌过滤
加有添加剂EDTA
Unit SizeEach
内容与储存
储存条件：15°C 至30°C
运输条件：室温
有效期：自生产之日起24个月

常见问题解答 (FAQ)

你们提供哪些用于解离细胞的试剂？这些产品之间的主要区别是什么？

请通过此选择列表(https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html)来比较我们的细胞解离试剂。

What is the osmolality and pH range for Cell Dissociation Buffer, enzyme-free, PBS (Cat. No. 13151014)

Below are QC test specifications for pH and osmolality for this product:

- pH: 6.9 to 7.9
- Osmolality: 289 to 321 mOsm/kg

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

At what confluency should cells be at prior to their dissociation using the Cell Association Buffer, enzyme-free, PBS?

The buffer should be used with cells that are sub-confluent (60-80%). If cells are more than 80% confluent, it will be hard to detach them.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will the Cell Dissociation Buffer, enzyme-free, PBS damage cell surface markers/receptors?

This buffer is very gentle. It should preserve the structural and functional integrity of cell surface proteins. However, we recommend that the customer test their cells and proteins.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I use the Cell Dissociation Buffer, enzyme-free, PBS to dissociate cells?

This Enzyme-Free Cell Dissociation Buffer does not rely on proteolysis for its action. Therefore, it is not suitable for strongly adherent cells, or for routine passage of some cell types (e.g., A431, Hep2, A549, MDCK, WI-38). This product is intended to gently dissociate cells from attachment substrates and each other while maintaining surface protein integrity.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all 细胞解离缓冲液，无酶，PBS FAQs

引用和文献 (3)

引用和文献
Abstract
Hematopoietic differentiation and production of mature myeloid cells from human pluripotent stem cells.
Authors:Choi KD, Vodyanik M, Slukvin II,
Journal:Nat Protoc
PubMed ID:21372811
'In this paper, we describe a protocol for hematopoietic differentiation of human pluripotent stem cells (hPSCs) and generation of mature myeloid cells from hPSCs through expansion and differentiation of hPSC-derived lin(-)CD34(+)CD43(+)CD45(+) multipotent progenitors. The protocol comprises three major steps: (i) induction of hematopoietic differentiation by coculture of hPSCs with OP9 ... More
Long term non-invasive imaging of embryonic stem cells using reporter genes.
Authors:Sun N, Lee A, Wu JC,
Journal:Nat Protoc
PubMed ID:19617890
'Development of non-invasive and accurate methods to track cell fate after delivery will greatly expedite transition of embryonic stem (ES) cell therapy to the clinic. In this protocol, we describe the in vivo monitoring of stem cell survival, proliferation and migration using reporter genes. We established stable ES cell lines ... More
Functional evidence for the mediation of diabetogenic T cell responses by HLA-A2.1 MHC class I molecules through transgenic expression in NOD mice.
Authors: Marron Michele P; Graser Robert T; Chapman Harold D; Serreze David V;
Journal:Proc Natl Acad Sci U S A
PubMed ID:12361980
Particular major histocompatibility complex (MHC) class II alleles clearly contribute to T cell-mediated autoimmune type 1 diabetes (T1D) in both humans and nonobese diabetic (NOD) mice. However, studies in NOD mice indicate MHC class I-restricted T cell responses are also essential to T1D development. In humans, epidemiological studies have suggested ... More