Feeder layer- and serum-free culture of human embryonic stem cells.
AuthorsAmit M, Shariki C, Margulets V, Itskovitz-Eldor J,
JournalBiol Reprod
PubMed ID14627547
In addition to their contribution to the research on early human development, human embryonic stem (hES) cells may also be used for cell-based therapies. Traditionally, these cells have been cultured on mouse embryonic fibroblast feeder layers, which allow their continuous growth in an undifferentiated state. However, the use of hES ... More