Protein kinase C betaII plays an essential role in dendritic cell differentiation and autoregulates its own expression.
AuthorsCejas PJ, Carlson LM, Zhang J, Padmanabhan S, Kolonias D, Lindner I, Haley S, Boise LH, Lee KP,
JournalJ Biol Chem
PubMed ID15917249
'Dendritic cells (DC) arise from a diverse group of hematopoietic progenitors and have marked phenotypic and functional heterogeneity. The signal transduction pathways that regulate the ability of progenitors to undergo DC differentiation, as well as the specific characteristics of the resulting DC, are only beginning to be characterized. We have ... More
Human olfactory epithelium in normal aging, Alzheimer's disease, and other neurodegenerative disorders.
AuthorsTrojanowski JQ, Newman PD, Hill WD, Lee VM,
JournalJ Comp Neurol
PubMed ID1723988
'By use of immunohistochemistry, we characterized the molecular phenotype of human olfactory epithelial (OE) cells and assessed the nature of the dystrophic olfactory neurites described initially in Alzheimer''s disease (AD). Keratin 8 was present in all classes of OE cells. Sustentacular cells lacked other cell type specific polypeptides and were ... More
Involvement of Ca2+-dependent PKCs in the adaptive changes of mu-opioid pathways to sympathetic denervation in the guinea pig colon.
AuthorsGiaroni C, Zanetti E, Pascale A, Oldrini R, Canciani L, Giuliani D, Amadio M, Chiaravalli AM, Lecchini S, Frigo GM,
JournalBiochem Pharmacol
PubMed ID19591807
'In the guinea pig colon, chronic sympathetic denervation entails supersensitivity to inhibitory mu-opioid agents modulating cholinergic neurons. The mechanism underlying such adaptive change has not yet been unravelled, although protein kinase C (PKC) may be involved. A previous study indirectly demonstrated that activation of mu-opioid receptors on myenteric neurons facilitates ... More
Distribution of gastrin-releasing peptide in the rat trigeminal and spinal somatosensory systems.
AuthorsTakanami K, Sakamoto H, Matsuda KI, Satoh K, Tanida T, Yamada S, Inoue K, Oti T, Sakamoto T, Kawata M,
Journal
PubMed ID24254931
Gastrin-releasing peptide (GRP) has recently been identified as an itch-specific neuropeptide in the spinal sensory system in mice, but there are no reports of the expression and distribution of GRP in the trigeminal sensory system in mammals. We characterized and compared GRP-immunoreactive (ir) neurons in the trigeminal ganglion (TG) with ... More
Genotype-selective phenotypic switch in primary afferent neurons contributes to neuropathic pain.
AuthorsNitzan-Luques A, Devor M, Tal M,
JournalPain
PubMed ID21872992
Pain is normally mediated by nociceptive Ad and C fibers, while Aß fibers signal touch. However, after nerve injury, Aß fibers may signal pain. Using a genetic model, we tested the hypothesis that phenotypic switching in neurotransmitters expressed by Aß afferents might account for heritable differences in neuropathic pain behavior. ... More
Neurons derived from a human teratocarcinoma cell line establish molecular and structural polarity following transplantation into the rodent brain.
AuthorsTrojanowski JQ, Mantione JR, Lee JH, Seid DP, You T, Inge LJ, Lee VM,
JournalExp Neurol
PubMed ID8405265
Studies of neurons grafted into the brains of experimental animals have been limited by the lack of suitably homogeneous populations of neurons for transplantation. Here we describe the transplantation and survival of pure, postmitotic human neurons (NT2N cells) into the rat brain. NT2N cells were derived from a human teratocarcinoma ... More
Amyloid plaques in cerebellar cortex and the integrity of Purkinje cell dendrites.
AuthorsLi YT, Woodruff-Pak DS, Trojanowski JQ,
JournalNeurobiol Aging
PubMed ID8159255
We probed serial and near serial sections of cerebellum from 13 Alzheimer's disease (AD), 10 older Down's syndrome (DS) patients, and 9 age-matched, non-AD controls, using single and double labeling immunohistochemistry to investigate the pathologic consequences of beta-amyloid or A4 (A beta) deposits in cerebellum and their relationship to Purkinje ... More
Characterization and differential distribution of the three major human protein kinase C isozymes (PKC alpha, PKC beta, and PKC gamma) of the central nervous system in normal and Alzheimer's disease brains.
AuthorsClark EA, Leach KL, Trojanowski JQ, Lee VM,
JournalLab Invest
PubMed ID1990207
Brain kinases may play important roles in normal memory as well as in the pathogenesis of neurodegenerative diseases. However, there is scant information on these enzymes in the human brain. For this reason, we characterized the immunohistochemical localization of protein kinase C (PKC) isozymes in human Alzheimer's disease (AD) and ... More