Primary human glomerular endothelial cells produce proteoglycans, and puromycin affects their posttranslational modification.
AuthorsBjörnson A, Moses J, Ingemansson A, Haraldsson B, Sörensson J,
JournalAm J Physiol Renal Physiol
PubMed ID15585670
This article describes the possible role of the endothelial cell-surface coat, containing proteoglycans (PGs) with connected glycosaminoglycans (GAGs), in maintaining glomerular permselectivity. Primary human glomerular endothelial cells (HGEC) in culture were treated with the nephrosis-inducing agent puromycin aminonucleoside (PAN). Analysis was made by TaqMan real-time PCR, Western blot analysis, and ... More
AuthorsStratman AN, Malotte KM, Mahan RD, Davis MJ, Davis GE,
JournalBlood
PubMed ID19822899
'We show that endothelial cell (EC)-generated vascular guidance tunnels (ie, matrix spaces created during tube formation) serve as conduits for the recruitment and motility of pericytes along EC ablumenal surfaces to facilitate vessel maturation events, including vascular basement membrane matrix assembly and restriction of EC tube diameter. During quail development, ... More
Fibroblast growth factor-2 during postnatal development of the tracheal basement membrane zone.
'Thickening of the basement membrane zone (BMZ) is a characteristic of several airway diseases; however, very little is known about how this process occurs. The purpose of this study was to define development of the BMZ in the trachea of growing rhesus monkeys at 1, 2, 3, and 6 mo ... More
Atypical development of the tracheal basement membrane zone of infant rhesus monkeys exposed to ozone and allergen.
'Development of the basement membrane zone (BMZ) occurs postnatally in the rhesus monkey. The purpose of this study was to determine whether house dust mite allergen (HDMA) plus ozone altered this process. Rhesus monkeys were exposed to a regimen of HDMA and/or ozone or filtered air for 6 mo. To ... More
Confluence switch signaling regulates ECM composition and the plasmin proteolytic cascade in keratinocytes.
AuthorsBotta A, Delteil F, Mettouchi A, Vieira A, Estrach S, Négroni L, Stefani C, Lemichez E, Meneguzzi G, Gagnoux-Palacios L,
JournalJ Cell Sci
PubMed ID22641690
In culture, cell confluence generates signals that commit actively growing keratinocytes to exit the cell cycle and differentiate to form a stratified epithelium. Using a comparative proteomic approach, we studied this 'confluence switch' and identified a new pathway triggered by cell confluence that regulates basement membrane (BM) protein composition by ... More
Perlecan up-regulation of FRNK suppresses smooth muscle cell proliferation via inhibition of FAK signaling.
We previously reported that fully assembled basement membranes are nonpermissive to smooth muscle cell (SMC) replication and that perlecan (PN), a basement membrane heparan sulfate proteoglycan, is a dominant effector of this response. We report here that SMC adhesion to basement membranes, and perlecan in particular, up-regulate the expression of ... More