The relative contribution exerted by AF-1 and AF-2 transactivation functions in estrogen receptor alpha transcriptional activity depends upon the differentiation stage of the cell.
AuthorsMérot Y, Métivier R, Penot G, Manu D, Saligaut C, Gannon F, Pakdel F, Kah O, Flouriot G,
JournalJ Biol Chem
PubMed ID15078875
'The activity of the transactivation functions (activation function (AF)-1 and AF-2) of the estrogen receptor alpha (ERalpha) is cell-specific. This study aimed to decipher the yet unclear mechanisms involved in this differential cell sensitivity, with particular attention to the specific influence that cell differentiation may have on these processes. Hence, ... More
Cadherin adhesion receptors orient the mitotic spindle during symmetric cell division in mammalian epithelia.
'Oriented cell division is a fundamental determinant of tissue organization. Simple epithelia divide symmetrically in the plane of the monolayer to preserve organ structure during epithelial morphogenesis and tissue turnover. For this to occur, mitotic spindles must be stringently oriented in the Z-axis, thereby establishing the perpendicular division plane between ... More
Recruitment of phosphoinositide 3-kinase defines a positive contribution of tyrosine kinase signaling to E-cadherin function.
AuthorsPang JH, Kraemer A, Stehbens SJ, Frame MC, Yap AS,
JournalJ Biol Chem
PubMed ID15556934
'Classical cadherin adhesion molecules can function as adhesion-activated cell-signaling receptors. One key target for cadherin signaling is the lipid kinase phosphoinositide (PI) 3-kinase, which is recruited to cell-cell contacts and activated by E-cadherin. In this study, we sought to identify upstream factors necessary for E-cadherin to activate PI 3-kinase signaling. ... More
Role of notch-1 and E-cadherin in the differential response to calcium in culturing normal versus malignant prostate cells.
AuthorsDalrymple S, Antony L, Xu Y, Uzgare AR, Arnold JT, Savaugeot J, Sokoll LJ, De Marzo AM, Isaacs JT,
JournalCancer Res
PubMed ID16230388
A panel of expression markers was validated and used to document that, when radical prostatectomy specimens are cultured in low (i.e., <260 micromol/L)-calcium (Ca2+)-serum-free, growth factor-defined (SFD) medium, what grows out are not prostatic cancer cells but basally derived normal transit-amplifying prostatic epithelial cells. The selective outgrowth of the normal ... More
EGFR signaling to p120-catenin through phosphorylation at Y228.
AuthorsMariner DJ, Davis MA, Reynolds AB,
JournalJ Cell Sci
PubMed ID14996911
Epidermal growth factor receptor (EGFR) signals to p120(ctn) (p120), implying a role for EGFR in modulating cell-cell adhesion in epithelial tissues. p120 controls cadherin turnover, and may have other roles that modulate cadherin adhesiveness. To clarify the role for EGFR and other tyrosine kinases in regulating p120 function, we have ... More
a-Catenin contributes to the strength of E-cadherin-p120 interactions.
AuthorsTroyanovsky RB, Klingelhöfer J, Troyanovsky SM
JournalMol Biol Cell
PubMed ID21937720
Cadherin-catenin interactions play an important role in cadherin-mediated adhesion. Here we present strong evidence that in the cadherin-catenin complex a-catenin contributes to the binding strength of another catenin, p120, to the same complex. Specifically, we found that a ß-catenin-uncoupled cadherin mutant interacts much more weakly with p120 than its full-size ... More