Tumor-suppressive maspin regulates cell response to oxidative stress by direct interaction with glutathione S-transferase.
AuthorsYin S, Li X, Meng Y, Finley RL, Sakr W, Yang H, Reddy N, Sheng S,
JournalJ Biol Chem
PubMed ID16049007
Maspin, a novel serine protease inhibitor, suppresses tumor progression in several cancer models, including an in vivo model for prostate cancer bone metastasis. However, the molecular mechanism of maspin remains illusive, primarily because its molecular targets are unknown. To this end, we used a full-length maspin cDNA bait to screen ... More
Raf kinase inhibitory protein regulates Raf-1 but not B-Raf kinase activation.
AuthorsTrakul N, Menard RE, Schade GR, Qian Z, Rosner MR,
JournalJ Biol Chem
PubMed ID15886202
Raf kinase inhibitory protein (RKIP; also known as phosphatidylethanolamine-binding protein or PEBP) is a modulator of the Raf/MAPK signaling cascade and a suppressor of metastatic cancer. Here, we show that RKIP inhibits MAPK by regulating Raf-1 activation; specifically, RKIP acts subsequent to Raf-1 membrane recruitment, prevents association of Raf-1 and ... More
Two heme binding sites are involved in the regulated degradation of the bacterial iron response regulator (Irr) protein.
AuthorsYang J, Ishimori K, O'Brian MR,
JournalJ Biol Chem
PubMed ID15613477
The iron response regulator (Irr) protein from Bradyrhizobium japonicum is a conditionally stable protein that degrades in response to cellular iron availability. This turnover is heme-dependent, and rapid degradation involves heme binding to a heme regulatory motif (HRM) of Irr. Here, we show that Irr confers iron-dependent instability on glutathione ... More
Connexin43 associated with an N-cadherin-containing multiprotein complex is required for gap junction formation in NIH3T3 cells.
AuthorsWei CJ, Francis R, Xu X, Lo CW,
JournalJ Biol Chem
PubMed ID15741167
Previous studies have indicated an intimate linkage between gap junction and adherens junction formation. It was suggested this could reflect the close membrane-membrane apposition required for junction formation. In NIH3T3 cells, we observed the colocalization of connexin43 (Cx43alpha1) gap junction protein with N-cadherin, p120, and other N-cadherin-associated proteins at regions ... More