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BLOCK-iT™ Fluorescent Oligo, for electroporation - FAQs

查看更多产品信息 BLOCK-iT™ Fluorescent Oligo, for electroporation - FAQs (13750062)

2 个常见问题解答

在Stealth RNAi siRNA实验中我应该使用什么阳性对照和阴性对照？

我们为Stealth siRNA提供多个阳性和阴性对照：

Stealth阳性对照有：
- Stealth RNAi siRNA Actin阳性对照（人）（货号12935141, 250 µL）
- Stealth RNAi siRNA Cyclophilin B阳性对照（人）（货号12935142, 250 µL）
- Stealth RNAi siRNA GAPDH阳性对照（人）（货号12935140, 250 µL）
- Stealth RNAi siRNA β-Lactamase报告基因对照（货号12935148, 250 µL）

- Stealth RNAi siRNA GFP报告基因对照（货号12935145, 250 µL）
- Stealth RNAi siRNA Luciferase报告基因对照（货号12935146, 250 µL）

Stealth阴性对照有：
提供高(55-65% GC), 中(45-55% GC), 或低(35-45% GC)三种GC含量的双链siRNA对照：
- 高GC含量的Stealth RNAi siRNA阴性对照（货号12935400, 250 µL）
- 高GC含量的Stealth RNAi siRNA阴性对照#2（货号12935114, 250 µL）
- 高GC含量的Stealth RNAi siRNA阴性对照#3（货号12935115, 250 µL）
- 中GC含量的Stealth RNAi siRNA阴性对照（货号12935300, 250 µL）
- 中GC含量的Stealth RNAi siRNA阴性对照#2（货号12935112, 250 µL）
- 中GC含量的Stealth RNAi siRNA阴性对照#3（货号12935113, 250 µL）
- 低GC含量的Stealth RNAi siRNA阴性对照（货号12935200, 250 µL）
- 低GC含量的Stealth RNAi siRNA阴性对照#2（货号12935110, 250 µL）
- 低GC含量的Stealth RNAi siRNA阴性对照#3（货号12935111, 250 µL）
- Stealth RNAi siRNA阴性对照试剂盒（货号12935100，包含每种GC含量阴性对照各20 µM各250 µL）
- 对于随机序列阴性对照，您可以使用BLOCK-iT RNAi Designer对您的siRNA或Stealth RNAi序列进行打乱。

您也可以使用我们的BLOCK-iT AlexaFluorRed Fluorescent Control（货号14750100）或BLOCK-iTFluorescent Oligo（货号2013, 13750062）作为转染的对照。

What are the positive and negative controls I should use with my Stealth RNAi siRNA experiment?

We offer several negative and positive controls for Stealth siRNA:

For Stealth Positive Control:
- Stealth RNAi siRNA GAPDH Positive Control (human) (Cat. No. 12935140, 250 µL)
- Stealth RNAi siRNA beta-Lactamase Reporter Control (Cat. No. 12935148, 250 µL)
- Stealth RNAi siRNA GFP Reporter Control (Cat. No. 12935145, 250 µL)
- Stealth RNAi siRNA Luciferase Reporter Control (Cat. No. 12935146, 250 µL)

For Stealth Negative Control:
Hi (55-65% GC), Med (45-55% GC), or Low (35-45% GC) Duplexes are offered:

- Stealth RNAi siRNA Negative Control Hi GC (Cat. No. 12935400, 250 µL)
- Stealth RNAi siRNA Negative Control Hi GC Duplex #2 (Cat. No. 12935114, 250 µL)
- Stealth RNAi siRNA Negative Control Hi GC Duplex #3 (Cat. No. 12935115, 250 µL)
- Stealth RNAi siRNA Negative Control Med GC Duplex (Cat. No. 12935300, 250 µL)
- Stealth RNAi siRNA Negative Control Med GC Duplex #2 (Cat. No. 12935112, 250 µL)
- Stealth RNAi siRNA Negative Control Hi GC Duplex #3 (Cat. No. 12935113, 250 µL)
- Stealth RNAi siRNA Negative Control Low GC Duplex (Cat. No. 12935200, 250 µL)
- Stealth RNAi siRNA Negative Control Low GC Duplex #2 (Cat. No. 12935110, 250 µL)
- Stealth RNAi siRNA Negative Control Low GC Duplex #3 (Cat. No. 12935111, 250 µL)
- Stealth RNAi siRNA Negative Control Kit (Cat. No. 12935100, contains one of each GC content level negative control, 250 µL each at 20 µM)
- For a scrambled negative control, you could also scramble your designed siRNA or Stealth RNAi sequence using the BLOCK-iT RNAi Designer.

You can also use our BLOCK-iT AlexaFluor Red Fluorescent Control (Cat. No. 14750100) or BLOCK-iT Fluorescent Oligo (Cat. No. 2013, 13750062) as a control for the transfection.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.