Preparation of retinal explant cultures to study ex vivo tip endothelial cell responses.
Authors:Sawamiphak S, Ritter M, Acker-Palmer A,
Journal:Nat Protoc
PubMed ID:20885378
This protocol details a culture technique for neonatal mouse retina that allows the assessment and quantification of acute responses of developing blood vessels to pharmacological manipulation. The technique has proven to be a useful tool for elucidating the molecular mechanisms that underlie the guidance of tip cells in the complex ... More
An evaluation of a four-channel multiplexed electrospray tandem mass spectrometry for higher throughput quantitative analysis.
Authors: Morrison Denise; Davies Amy E; Watt Alan P;
Journal:Anal Chem
PubMed ID:11985324
A four-channel multiplexed electrospray inlet system (MUX) coupled to a triple quadrupole mass spectrometer was investigated as a higher throughput approach to quantitative analysis. Four discrete samples may be simultaneously analyzed by virtue of a rotating sampler with a concomitant 4-fold increase in analytical throughput. Although absolute sensitivity was reduced ... More