HBSS (10X), calcium, magnesium, no phenol red
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HBSS (10X), calcium, magnesium, no phenol red
Gibco™

HBSS (10X), calcium, magnesium, no phenol red

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation,Read more
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Catalog NumberQuantity
14065056500 mL
Catalog number 14065056
Price (CNY)
649.20
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Ends: 31-Dec-2025
827.00
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Quantity:
500 mL
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Price (CNY)
649.20
Online Exclusive
Ends: 31-Dec-2025
827.00
Save 177.80 (21%)
Each
Add to cart

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations with calcium and magnesium are generally used as transport media, or for reagent preparation. Concentrated forms of Gibco™ HBSS require sodium bicarbonate addition, pH adjustment, and filtration at the time of preparation (see protocol for details).

We offer a variety of Gibco™ HBSS formulations for a range of cell culture applications.


This HBSS is modified as follows:
WithWithout
• Calcium• Phenol Red
• Magnesium• Sodium Bicarbonate
• Glucose 


The complete formulation is available.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Chemical Name or MaterialHanks' Balanced Salt Solution (HBSS)
IncludesNo Sodium Pyruvate
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Osmolality2700 - 3070 mOsm/kg
Recommended StorageStorage conditions: 15°C to 30°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture
SterilitySterile-filtered
Concentration10 X
For Use With (Application)Variety of Cell Culture Applications; Such as Washing Cells Before Dissociation; Transporting Cells or Tissue Samples; Diluting Cells for Counting And Preparing Reagents
Physical FormLiquid
Product LineGibco™
Quantity500 mL
Solution TypeHank's Balanced Salt
pH4.4 - 4.7
Unit SizeEach

Frequently asked questions (FAQs)

Can I store Hank's Balanced Salt Solution (HBSS) in the refrigerator?

The recommended storage condition for our HBSS products is at 15-30 degrees C. Generally, storage at 4 degrees C will not impact these products. The only concern would be for the 10x HBSS solutions with calcium and magnesium as these salts might precipitate when stored outside the recommended temperature ranges. As long as you don't see any precipitation upon refrigeration, the product will be fine.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I understand that some media are worse than others for fluorescence imaging. How do I choose?

Most media contain phenol red, which can quench fluorescent dyes in the visible wavelengths. Most media also contain autofluorescent components, such as riboflavin, which can reduce signal-to-background. We offer FluoroBrite DMEM and HEPES-based Live Cell Imaging Solution, which have been optimized for fluorescent imaging. We also offer a number of media without phenol red. But if none of these are reasonable options for your experiment, then we also offer BackDrop Background Suppressor ReadyProbes Reagent, which can be added to quench media autofluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Should I be concerned about phenol red in my media when labeling my live cells with fluorescent dyes?

Some cell types accumulate phenol red, and this can pose a problem in the use of many fluorescent probes. Phenol red can quench visible-wavelength dyes and, although phenol red is non-fluorescent, various impurities may be fluorescent. We have many phenol red-free media to choose from. Our Live Cell Imaging Solution (HEPES-based) and our FluoroBrite DMEM have been optimized to be phenol red-free as well as to be non-autofluorescent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you offer media without phenol red (phenol red-free media)?

Yes, please visit this page for a list of our phenol red-free media (http://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.