12 Well Carrier Plate System, no inserts, Pack of 1 - FAQs

查看更多产品信息 Nunc™ Cell Culture Inserts in Carrier Plate Systems - FAQs (141086, 141078, 141002, 141004, 141006, 141008, 141080, 141082)

4 个常见问题解答

With the Nunc Polycarbonate Cell Culture Inserts in Multi-Well Plates, what is the distance between the cell culture membrane and the bottom of the multi-well dish?

The membrane is approximately 1.0 mm above the bottom of the dish for the Nunc Polycarbonate Cell Culture Inserts.

Can I observe cells on the cell culture membrane when the Nunc Polycarbonate Cell Culture Insert is inside of a multi-well plate?

Due to the large pore size of the Nunc polycarbonate inserts, spots or holes are observed and they represent the membrane. In this plane of focus, the most visible cells are the ones located on the membrane, whereas the slightly blurred backdrop are the cells on the dish that are in a different plane of focus. These cells do not photograph with great resolution due to the slightly opaque quality of the membrane. However, these cells are visible in culture.

What is the recommended Multidish chamber media volume per carrier plate insert and height position?

Low position: 2 mL (12-well), 1 mL (24-well), Medium position: 3 mL (12-well), 1.5 mL (24-well), High position: 4 mL (12-well), 2 mL (24-well). Some optimization may be necessary for development purposes.

What is the difference between your cell culture membrane inserts and carrier plates?

Standard culture membrane inserts are placed directly into the wells of a Multidish, and the feet at the bottom of the insert maintain a distance of about 1 mm between the membrane and the Multidish culture surface. The “carrier plate” culture membrane product adds a tray between the Multidish and the lid, with a set of hanger brackets at each well. These brackets engage one of three sets of tabs on each insert, to allow for selection between 3 different distances (approximately 1, 3, or 6 mm) between the membrane and the Multidish culture surface. This can allow for more flexibility in experiments, such as distance-dependent interaction between populations or media volume under cultures at the air-media interface.