DPBS，无钙、无镁

DPBS, no calcium, no magnesium, 10x 500 mL (Cat. Nos. 14190250 and 14190169) have the same formulation. The only difference is in the manufacturing site.

The DPBS, no calcium, no magnesium (Cat. no. 14190169) is manufactured in our Inchinnan, Scotland facility and is intended for sale in Europe, Africa, and the Middle East—higher freight charges will apply to orders shipped elsewhere. DPBS, no calcium, no magnesium (Cat. No. 14190250) is manufactured in Grand Island, US, and is recommended for our customers in North America, Latin America, and the Asia/Pacific region.

The complete formulation can be found here:

14190 - DPBS, no calcium, no magnesium

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We cannot provide an official expiration date for open bottles as this depends on the customer's use. Once opened, Thermo Fisher Scientific can no longer guarantee the performance of any product. However, if the storage conditions are followed as recommended, the stated expiration date (36 months from the date of manufacture) should apply to opened bottles as DPBS is extremely stable.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

DPBS has a shelf life of 36 months from the date of manufacture, when stored as recommended (15-30 degrees C).

The basal Diploid Growth Serum-Reduced Medium already contains 6 mM L-glutamine.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

We recommend evaluating performance with Diploid Production Serum-Free Medium using current conditions, however, multiplicity of infection, time of infection, and time of harvest may be different than with conventional media. Closely monitor cells for cytopathic effect and evaluate viral titers at multiple time points.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.