DPBS, no calcium, no magnesium, 5 L bag - FAQs

有的。尽管我们感觉PE膜材料在技术性能上超越了市面上许多其他膜材料，我们仍将以订购的形式继续供应和支持我们目前正在销售的其他膜材料。

我们对每一尺寸的培养袋都设计了特定重量的瓦楞纸箱来辅助运输：
5 L: 12” x 14.25” x 5.5”
10 L: 13” x 23.5” x 6.125”
20 L: 15” x 24” x 6.125”  

这一市销的膜材料是一种非常透明，多层的单卷膜材料，并拥有聚乙烯接触表面，可制成各种不同的尺寸（250 mL至1000 L）。此外，这一膜材料还具有卓越的气体阻隔性能和极低渗漏率，是市场的领先产品。点击此处 了解更多关于PE膜材料的说明。

基于用户的反馈，我们设计了一个袋装培养基的系列，所有尺寸的培养袋均使用一致的膜体进行包装。这一膜材料——称为PE膜——比其他市售膜材料的功能更优。点击此处了解更多关于PE膜材料的说明。

Gibco细胞培养袋（Cell Culture Bag）是预先灌注培养基，使用方便的包装，具有多种配方与体积，特别针对提升效能与降低成本的需求进行了专门设计。这些培养袋的体积可达1000升。请点击此处阅读更多关于这些培养袋的相关信息。

We cannot provide an official expiration date for open bottles as this depends on the customer's use. Once opened, Thermo Fisher Scientific can no longer guarantee the performance of any product. However, if the storage conditions are followed as recommended, the stated expiration date (36 months from the date of manufacture) should apply to opened bottles as DPBS is extremely stable.

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DPBS has a shelf life of 36 months from the date of manufacture, when stored as recommended (15-30 degrees C).

The basal Diploid Growth Serum-Reduced Medium already contains 6 mM L-glutamine.

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We recommend evaluating performance with Diploid Production Serum-Free Medium using current conditions, however, multiplicity of infection, time of infection, and time of harvest may be different than with conventional media. Closely monitor cells for cytopathic effect and evaluate viral titers at multiple time points.

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Diploid Growth Serum-Reduced Medium can be supplemented with Fetal Bovine Serum, Newborn Calf Serum, Donor Bovine Serum with Iron, or Bovine Serum.

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Diploid Growth Serum-Reduced Medium has been evaluated with fibroblast cell lines, including MRC-5, WI-38, IMR-90, BS-2, and CEF cells.

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Once supplemented, the complete Diploid Growth Serum-Reduced Medium must be used within 4 weeks.

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Most diploid cell lines can be frozen and thawed in Diploid Growth Serum-Reduced Medium. Consult the Diploid Growth Serum Reduced Medium User Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0019015_DiploidGrowthSerumReducedMedium_UG.pdf) for additional information.

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Cells growing in medium with serum can be transferred directly to Diploid Growth Serum-Reduced Medium without adaptation. Continue to monitor and passage cells for 2-3 passages until consistent growth is achieved. Cultures may benefit from supplementation with 1-2% serum.

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Sorry, we do not test the conductivity of our DPBS products and hence cannot provide a value or range.

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Our DPBS products are for cell culture use and have not been tested for DNase/RNase activity. We do not recommend using these products for RNA work.

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Yes. Although we feel that the PE film exceeds many of the technical properties of the other available films, we will continue to offer and support our other currently available films through custom orders.

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Each bag size will arrive in a specific heavyweight corrugated box designed for shipment:
5 L: 12” x 14.25” x 5.5”
10 L: 13” x 23.5” x 6.125”
20 L: 15” x 24” x 6.125”

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The commercially available film is a very clear, multi-layered, single web film with a polyethylene contact surface that can be made into a wide range of bag sizes (250 mL to 1,000L). In addition, the film is a market leader with exceptional gas barrier properties and very low extractable and leachable results. Click here to read more about the PE Film specifications (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/classical-media/gibco-cell-culture-bags.html).

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Based on feedback from customers, we designed a media bag portfolio that uses a consistent film for all bag sizes. This film, called PE film, is superior in function to other commercially available films. Please go here to read more about the PE Film specifications (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/classical-media/gibco-cell-culture-bags.html).

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Gibco Cell Culture Bags are convenient, pre-filled bags in a variety of formulations and sizes, specifically designed to increase productivity and reduce costs. The bags can be formatted up to 1,000 L. Read more about these bags here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/classical-media/gibco-cell-culture-bags.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.