What is the shelf life of DPBS after it has been opened?
We cannot provide an official expiration date for open bottles as this depends on the customer's use. Once opened, Thermo Fisher Scientific can no longer guarantee the performance of any product. However, if the storage conditions are followed as recommended, the stated expiration date (36 months from the date of manufacture) should apply to opened bottles as DPBS is extremely stable.
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What is the shelf life of DPBS (Cat. No. 14190136)?
DPBS has a shelf life of 36 months from the date of manufacture, when stored as recommended (15-30 degrees C).
Does Diploid Growth Serum-Reduced Medium (SRM) contain L-glutamine?
The basal Diploid Growth Serum-Reduced Medium already contains 6 mM L-glutamine.
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Do I have to adjust my infection parameters when using Diploid Production Serum-Free Medium (SFM)?
We recommend evaluating performance with Diploid Production Serum-Free Medium using current conditions, however, multiplicity of infection, time of infection, and time of harvest may be different than with conventional media. Closely monitor cells for cytopathic effect and evaluate viral titers at multiple time points.
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Can Diploid Growth Serum-Reduced Medium (SRM) be supplemented with different types of serum?
Diploid Growth Serum-Reduced Medium can be supplemented with Fetal Bovine Serum, Newborn Calf Serum, Donor Bovine Serum with Iron, or Bovine Serum.
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