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DPBS (10X)，无钙、无镁
引用和文献 (1)
Gibco™

DPBS (10X)，无钙、无镁

Dulbecco 磷酸盐缓冲溶液 (DPBS) 是一种平衡盐溶液，适用于各种细胞培养应用，例如解离前清洗细胞、运输细胞或组织样品、稀释细胞进行计数和制备试剂。在细胞解离之前，需采用不含钙和镁的制剂从培养物中冲洗掉螯合剂。浓缩液形式的 Gibco™ DPBS了解更多信息
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货号数量
14200075500 mL
1420016610 x 500 mL
货号 14200075
价格（CNY)
698.00
飞享价
Ends: 31-Dec-2026
873.00
共减 175.00 (20%)
Each
数量:
500 mL
Customize this product
价格（CNY)
698.00
飞享价
Ends: 31-Dec-2026
873.00
共减 175.00 (20%)
Each
Dulbecco 磷酸盐缓冲溶液 (DPBS) 是一种平衡盐溶液，适用于各种细胞培养应用，例如解离前清洗细胞、运输细胞或组织样品、稀释细胞进行计数和制备试剂。在细胞解离之前，需采用不含钙和镁的制剂从培养物中冲洗掉螯合剂。浓缩液形式的 Gibco™ DPBS 在配制时需要调整 pH 和过滤（详见实验方案）。

我们针对大量细胞培养应用提供了各种 Gibco™ DPBS 制剂。

DPBS 的改良方式如下：

不含
•钙
•镁离子
• 酚红

可提供完整配方。

cGMP 生产和质量体系
Gibco™ DPBS 在位于英国苏格兰佩斯利、符合 cGMP 要求的工厂生产。该工厂是在 FDA 注册的医疗器械生产商，且通过 ISO 13485 标准认证。为确保供应链的稳定，我们同时提供由我们格兰德岛工厂生产的等同 Gibco™ DPBS 产品 (14200-075)。本工厂者亦是在 FDA 登记的医疗器械生产商，且符合 ISO 13485 标准。
仅用于研究和生产用途。不可用于临床诊断或直接用于人类或动物。
规格
化学名称或材料Dulbecco 的磷酸盐缓冲液 (DPBS)
颜色清除
包括不含丙酮酸钠
生产质量cGMP-compliant under the ISO 13485 standard
渗透压2630 - 3000 mOsm/kg
建议的储存条件储存条件:15°C 至 30°C, 运输条件:室温, 有效期:自生产之日起 36 个月
无菌无菌过滤
灭菌方法Sterile-filtered
最大浓度10 X
适用于（应用）哺乳动物细胞培养
分级Cell Culture
物理形态液体
产品线Gibco
数量500 mL
溶液类型Dulbecco 的磷酸盐缓冲液
pH6.7 - 7.0
Unit SizeEach

常见问题解答 (FAQ)

Do you offer an alternative for the discontinued 3-Germ Layer ICC Kit (Cat. No. A25538)?

We do not have a direct alternative for the discontinued 3-Germ Layer ICC Kit (Cat. No. A25538). However, we do have alternative primary and secondary antibodies, as well as reagents, that can be used for trilineage differentiation. Please note that we have not internally validated the use of all these reagents together.

We recommend the following primary antibodies:

Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4 (asm-1)) (Cat. No. MA5-11547)

alpha-Fetoprotein Monoclonal Antibody (AFP3) (Cat. No. 14-6583-80)

beta-3 Tubulin Monoclonal Antibody (2G10) (Cat. No. MA1-118)

We recommend the following secondary antibodies:

Goat anti-Mouse IgG2a Cross-Adsorbed, Alexa Fluor 555 (Cat. No. A21137)

Goat anti-Mouse IgG2a Cross-Adsorbed, Alexa Fluor 594 (Cat. No. A21135)

https://www.thermofisher.com/antibody/product/Goat-anti-Mouse-IgG1-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A-21121

We recommend using the following reagents:

https://www.thermofisher.com/order/catalog/product/88-8824-00

NucBlue Fixed Cell ReadyProbes Reagent (DAPI) (Cat. No. R37606)

Blocker BSA (Cat. No. 37520)

DPBS (10X), no calcium, no magnesium (Cat. No. 14200075)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I get a specific value for the conductivity for your DPBS products?

Sorry, we do not test the conductivity of our DPBS products and hence cannot provide a value or range.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are your DPBS products DNase/RNase-free? Can I use them for RNA work?

Our DPBS products are for cell culture use and have not been tested for DNase/RNase activity. We do not recommend using these products for RNA work.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

引用和文献 (1)

引用和文献
Abstract
Isolation of multipotent neural stem or progenitor cells from both the dentate gyrus and subventricular zone of a single adult mouse.
Authors:Guo W, Patzlaff NE, Jobe EM, Zhao X,
Journal:Nat Protoc
PubMed ID:23080272
In adult mammals, the subventricular zone (SVZ) of the lateral ventricles and the subgranular zone of the dentate gyrus (DG) show ongoing neurogenesis, and multipotent neural stem or progenitor cells (NSCs) in these two regions exhibit different intrinsic properties. However, investigation of the mechanisms underlying such differences has been limited ... More