Monitoring and contamination incidence of gnotobiotic experiments performed in microisolator cages.
AuthorsBasic M, Bolsega S, Smoczek A, Gläsner J, Hiergeist A, Eberl C, Stecher B, Gessner A, Bleich A
JournalInt J Med Microbiol
PubMed ID33636479
'With the increased interest in the microbiome research, gnotobiotic animals and techniques emerged again as valuable tools to investigate functional effects of host-microbe and microbe-microbe interactions. The increased demand for gnotobiotic experiments has resulted in the greater need for housing systems for short-term maintenance of gnotobiotic animals. During the last ... More
CircINSR Regulates Fetal Bovine Muscle and Fat Development.
AuthorsShen X, Tang J, Ru W, Zhang X, Huang Y, Lei C, Cao H, Lan X, Chen H
JournalFront Cell Dev Biol
PubMed ID33490079
'The level of muscle development in livestock directly affects the production efficiency of livestock, and the contents of intramuscular fat (IMF) is an important factor that affects meat quality. However, the molecular mechanisms through which circular RNA (circRNA) affects muscle and IMF development remains largely unknown. In this study, we ... More
Dynamic regulation of connexins in stem cell pluripotency.
'Characterization of the pluripotent "ground state" has led to a greater understanding of species-specific stem cell differences and has imparted an appreciation of the pluripotency continuum that exists in stem cells in vitro. Pluripotent stem cells are functionally coupled via connexins that serve in gap junctional intercellular communication (GJIC) and ... More
Four high-quality draft genome assemblies of the marine heterotrophic nanoflagellate Cafeteria roenbergensis.
'The heterotrophic stramenopile Cafeteria roenbergensis is a globally distributed marine bacterivorous protist. This unicellular flagellate is host to the giant DNA virus CroV and the virophage mavirus. We sequenced the genomes of four cultured C. roenbergensis strains and generated 23.53?Gb of Illumina MiSeq data (99-282?×?coverage per strain) and 5.09?Gb of ... More
Generation of an induced pluripotent stem cell line (TRNDi008-A) from a Hunter syndrome patient carrying a hemizygous 208insC mutation in the IDS gene.
AuthorsHong J, Xu M, Li R, Cheng YS, Kouznetsova J, Beers J, Liu C, Zou J, Zheng W
JournalStem Cell Res
PubMed ID31071499
'Mucopolysaccharidosis Type II (MPS II), also known as Hunter syndrome, is a rare X-linked genetic disease caused by mutations in the IDS gene encoding iduronate 2-sulfatase (I2S). This is a multisystem disorder with significant variation in symptoms. Here, we document a human induced pluripotent stem cell (iPSC) line generated from ... More
Myeloid-Derived Suppressor Cells Produce IL-10 to Elicit DNMT3b-Dependent IRF8 Silencing to Promote Colitis-Associated Colon Tumorigenesis.
AuthorsIbrahim ML, Klement JD, Lu C, Redd PS, Xiao W, Yang D, Browning DD, Savage NM, Buckhaults PJ, Morse HC, Liu K
JournalCell Rep
PubMed ID30540937
IL-10 functions as a suppressor of colitis and colitis-associated colon cancer, but it is also a risk locus associated with ulcerative colitis. The mechanism underlying the contrasting roles of IL-10 in inflammation and colon cancer is unknown. We report here that inflammation induces the accumulation of CD11b ... More
Morphological and molecular description of Sarcocystis ratti n. sp. from the black rat (Rattus rattus) in Latvia.
AuthorsPrakas P, Kirillova V, Gavarane I, Gravele E, Butkauskas D, Rudaityte-Lukošiene E, Kirjušina M
JournalParasitol Res
PubMed ID31297600
Rodents have been widely studied as intermediate hosts of Sarcocystis; however, only a few reports on these parasites in the black rat (Rattus rattus) are known. Having examined 13 black rats captured in Latvia, sarcocysts were found in skeletal muscles of two mammals and were described as Sarcocystis ratti n. ... More
miR-21 inhibitor facilitates the anticancer activity of doxorubicin loaded nanometer in melanoma.
AuthorsWei X, You X, Zhang J, Zhou C
JournalOncol Rep
PubMed ID31115580
MicroRNA-21 (miR-21) is a potential therapeutic target for melanoma. Whether miR-21 inhibitor affects the anti-cancer activity of doxorubicin assisted by c(RGDyK)-modified liposome (DLN) in melanoma and the underlying mechanisms are largely unknown. In this study, in vitro and animal models were used to explore the effect of DLN combined with ... More
Divergent genes encoding the putative receptors for growth hormone and prolactin in sea lamprey display distinct patterns of expression.
AuthorsGong N, Ferreira-Martins D, McCormick SD, Sheridan MA
JournalSci Rep
PubMed ID32015405
Growth hormone receptor (GHR) and prolactin receptor (PRLR) in jawed vertebrates were thought to arise after the divergence of gnathostomes from a basal vertebrate. In this study we have identified two genes encoding putative GHR and PRLR in sea lamprey (Petromyzon marinus) and Arctic lamprey (Lethenteron camtschaticum), extant members of ... More
The DUF328 family member YaaA is a DNA-binding protein with a novel fold.
AuthorsPrahlad J, Yuan Y, Lin J, Chang CW, Iwata-Reuyl D, Liu Y, de Crécy-Lagard V, Wilson MA
JournalJ Biol Chem
PubMed ID32796037
DUF328 family proteins are present in many prokaryotes; however, their molecular activities are unknown. The
Development of a species-specific transformation system using the novel endogenous promoter calreticulin from oleaginous microalgae Ettlia sp.
AuthorsLee JW, Lee MW, Ha JS, Kim DS, Jin E, Lee HG, Oh HM
JournalSci Rep
PubMed ID32811857
Microalgae not only serve as raw materials for biofuel but also have uses in the food, pharmaceutical, and cosmetic industries. However, regulated gene expression in microalgae has only been achieved in a few strains due to the lack of genome information and unstable transformation. This study developed a species-specific transformation ... More
Comprehensive Genome-Wide Exploration of C2H2 Zinc Finger Family in Grapevine (
AuthorsArrey-Salas O, Caris-Maldonado JC, Hernández-Rojas B, Gonzalez E
JournalGenes (Basel)
PubMed ID33672655
Some C2H2 zinc-finger proteins (ZFP) transcription factors are involved in the development of pollen in plants. In grapevine (
Maternal inheritance of mitochondrial DNA in mice after inter-species hybridization and 138 generations of backcrossing.
AuthorsWharton D, Morey KC, Hanner R
JournalMitochondrial DNA A DNA Mapp Seq Anal
PubMed ID33416414
Investigating the distribution of strains of Erwinia amylovora and streptomycin resistance in apple orchards in New York using CRISPR profiles: a six-year follow-up.
AuthorsWallis A, Yannuzzi IM, Choi MW, Spafford J, Siemon M, Ramachandran P, Timme R, Pettengill J, Cagle R, Ottesen AR, Cox K
JournalPlant Dis
PubMed ID33599513
Fire blight, caused by the bacterium
Limited antimicrobial efficacy of oral care antiseptics in microcosm biofilms and phenotypic adaptation of bacteria upon repeated exposure.
AuthorsSchwarz SR, Hirsch S, Hiergeist A, Kirschneck C, Muehler D, Hiller KA, Maisch T, Al-Ahmad A, Gessner A, Buchalla W, Cieplik F
JournalClin Oral Investig
PubMed ID33033920
The aims of this study were to investigate the antimicrobial efficacy of antiseptics in saliva-derived microcosm biofilms, and to examine phenotypic adaption of bacteria upon repeated exposure to sub-inhibitory antiseptic concentrations. ... More