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View additional product information for Trypsin (0.25%), phenol red - FAQs (15050065, 15050057)
10 product FAQs found
通常情况下，胰酶可在冰箱中保存一周。不过，这一时间长度也随着这一周内的加热次数和使用频率而变化。同时，它也与细胞粘附的紧密程度有关，胰酶在非冰冻条件下每天都会丧失一定的活性。
TrypLE试剂不含动物源性与人源成份，纯度极高且对细胞的作用十分温和。该产品无需使用胰酶抑制剂对其进行终止。在含血清和不含血清的培养条件下，TrypLE试剂都是解离各类贴壁培养的哺乳动物细胞系的理想之选，能够直接做为胰酶替代品而应用于您当前的实验方案。
TrypLE试剂在常温下十分稳定，可随时按需取用。TrypLE细胞解离试剂能够在室温下稳定保存24个月，储存与操作都更为容易方便。请通过此选择列表(https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html)来比较我们的细胞解离试剂。
是的，请访问此页面(https://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf)浏览我们的无酚红培养基页面。
Generally speaking, trypsin can be stored refrigerated for up to a week. However, this may vary depending on how often it is warmed up and used during that week. It may also depend on how tightly the cells are attached, because there will be some loss of activity each day the trypsin is not frozen.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Yes, the TrypLE products contain rProtease, a non-animal, trypsin-like enzyme used for the dissociation of attachment dependent cell lines. TrypLE enzyme has demonstrated the ability to dissociate cells cultured both in serum-free and serum-supplemented systems. The catalog numbers for TrypLE Select are 12563-011 (100 mL) and 12563-029 (500 mL). Some example catalog numbers for TrypLE Express include 12604-013 without Phenol red (100 mL) and 12605-010 with Phenol red (100 mL). Both formulations are also available in additional sizes. All these products are animal origin-free.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
All the above products contain rProtease, a non-animal, trypsin-like enzyme formulation used for the dissociation of attachment-dependent cell lines. This alternative for porcine trypsin is a recombinant enzyme derived from microbial fermentation.
The TrpLE Express, TrypLE Select, and CTS TrypLE Select products differ in their production and testing methods and in the documentation provided.
TrypLE Select is for the industrial market and has release-testing both for endotoxins and enzyme activity. This material also has a Drug Master File on record. It is manufactured in AOF-dedicated equipment and is intended for Research Use/Further Cell Culture Manufacturing.
TrypLE Express is manufactured without use of dedicated equipment and is intended for Research Use Only. Not for use in diagnostic procedures.
CTS TrypLE Select is manufactured under scalable cGMP conditions using dedicated equipment and is designed for clinical applications. This product has a Drug Master File on record and is intended for Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. Not intended for direct administration into humans or animals.
To read more about TrypLE and cell release times using this reagent, please go here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/tryple-express.html#1).
Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.
TrypLE reagent is free of animal- and human-derived components, producing an exceptionally pure reagent that is gentle on cells. Inactivation with trypsin inhibitors is not required. TrypLE reagent is ideal for dissociating a variety of attachment-dependent mammalian cell lines both in serum and in serum-free conditions, and can be directly substituted for trypsin in your current protocol. TrypLE reagent is room-temperature stable and ready to use when you need it. TrypLE cell dissociation reagents remain stable for 24 months at room temperature, making storage and handling easier and more convenient.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).
Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.
Yes, please visit this page for a list of our phenol red-free media (http://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf).
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.