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查看更多产品信息 Pierce™ Glutathione Coated Plates - FAQs (15340, 15240, 15140)
8 个常见问题解答
Penicillin interferes with the final stages of bacterial cell wall synthesis.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
The background is extremely low when using the Pierce Glutathione Coated Plates. This results in a high signal-to-noise ratio and a low detection limit which in general means that the detection limit (OD 0.5) is 3 ng per well (100 µL).
The Pierce Glutathione Coated Plates are extremely stable over long periods of time, if stored at room temperature (20-25 degrees C). We guarantee 12 mths of shelf life in unopened foil package after the date of shipment.
To determine the approximate concentration of a GST-tagged protein, a standard curve should be generated using purified GST added in different concentrations to the wells of the plates. Please note that it is only the approximately concentration that can be determined since not 100% of the GST-fusion proteins will be bound due to denaturing or steric hindrance to GSH.
No. Do not whirl-mix a solution containing GST-tagged proteins. The CV will increase due to slight degradation of the GST protein.
We recommend using 0.05% Tween-20 surfactant (as the non-ionic detergent) to improve the signal-to-noise ratio of the assay and thus decrease the background.
Yes. We recommend using a solution of the purified GST or GST-tagged protein diluted in Phosphate Buffered Saline (PBS), pH 7.2 in a concentration ranging from 0.01-1 µg/mL. During the coupling, non-ionic detergents like Tween-20 surfactant should not be present, as these will suppress the coupling.
The Pierce Glutathione Coated Plates are designed for optimal detection of glutathione-S-transferase (GST)-tagged proteins or purified GST.