QuantaRed™ 增强型化学荧光 HRP 底物试剂盒
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QuantaRed™ 增强型化学荧光 HRP 底物试剂盒
Thermo Scientific™

QuantaRed™ 增强型化学荧光 HRP 底物试剂盒

Thermo Scientific QuantaRed 增强型化学荧光 HRP 底物 (ADHP) 是最灵敏的荧光 ELISA 底物,可提供与增强型化学发光相当的灵敏度了解更多信息
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货号数量
15159110 ml
货号 15159
价格(CNY)
5,137.00
飞享价
Ends: 31-Dec-2025
6,738.00
共减 1,601.00 (24%)
Each
添加至购物车
数量:
110 ml
请求批量或定制报价
价格(CNY)
5,137.00
飞享价
Ends: 31-Dec-2025
6,738.00
共减 1,601.00 (24%)
Each
添加至购物车
Thermo Scientific QuantaRed 增强型化学荧光 HRP 底物 (ADHP) 是最灵敏的荧光 ELISA 底物,可提供与增强型化学发光相当的灵敏度。该试剂盒含有 QuantaRed ADHP 浓缩液、QuantaRed 增强剂溶液、QuantaRed 稳定过氧化物溶液和 QuantRed 终止液,足以用于 960 孔(10 x 96 孔板,100 µL 工作溶液/孔)。

QuantaRed 增强型化学荧光 HRP 底物试剂盒的特点:

灵敏 — 低皮克级至飞克级检测
红移 - 来自生物样品的自发荧光干扰极小
便利 — 与现有滤光片组兼容,无需购买特殊滤光片组
整套提供 — 混合并使用试剂,无需制备缓冲液或溶解试剂
灵活 — 使用终止液,以有效终止反应,获得至少 4 小时或更长时间的稳定信号。
通用 — 产生较强的比色信号,在 570 nm 处读取荧光或比色信号

QuantaRed HRP 底物可以 ELISA 规格对过氧化物酶活性进行高效、灵敏的荧光和比色检测。QuantaRed 底物使用专利技术,以提高充分记录的 ADHP(10-乙酰基-3,7-二羟基吩噁嗪)化学荧光反应的荧光得率和灵敏度。ADHP 是一种非荧光化合物,与辣根过氧化物酶 (HRP) 反应生成试卤灵,一种可溶性高荧光反应产物(最大激发/发射波长 ∼570/585 nm)。试卤灵的长波长发射使来自低波长(蓝色和绿色)自发荧光的干扰降至最低,使其成为对生物样品中的靶标分子进行定量测定的理想底物。此外,QuantaRed 底物的反应产物具有比色性和荧光性,可在 ∼570 nm 处通过分光光度法进行读数。

专门配制的 QuantaRed 底物含有化学增强剂,具有较大灵敏度和优异的低端线性。QuantaRed 增强型 HRP 底物具有较高的量子产率和较大的消光系数,灵敏度与增强型化学发光相当,在 ELISA 应用中检测 HRP 时比 Amplex 红色底物更灵敏。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
产品线QuantaRed™
底物属性化学底物
底物类型HRP(辣根过氧化物酶)
产品类型ELISA 底物
数量110 ml
Unit SizeEach
内容与储存
足够用于:960 孔(10 x 96 孔板),100 μL 工作溶液/孔
•QuantaRed ADHP 浓缩液,1 mL
• QuantaRed 增强剂溶液,50 mL
• QuantaRed 稳定过氧化物溶液,50 mL
• QuantaRed 终止液,10 mL

在 4°C 下储存。

常见问题解答 (FAQ)

Can QuantaRed Enhanced Chemifluorescent HRP Substrate be used for Western blotting?.

No. This is a soluble HRP substrate for ELISA and other plate-based applications.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What antibody concentrations are recommended for use with QuantaRed Enhanced Chemifluorescent HRP Substrate?

The recommended concentrations of antibodies and HRP conjugate are listed below:

Capture Antibody: 1-10 µg/mL
Detection Antibody: 0.05-1 µg/mL
HRP Conjugate: 0.1-0.2 µg/mL

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can a stop solution from another source be used with the QuantaRed Enhanced Chemifluorescent HRP Substrate?

No. The QuantaRed Stop Solution included in the kit must be used to effectively stop the reaction.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can the QuantaRed Enhanced Chemifluorescent HRP Substrate signal be measured in a kinetic mode without stopping the reaction?

To measure in a kinetic mode, simply omit the Stop Solution. At high HRP concentrations and prolonged reaction times, the fluorescent product may self-quench, which is observed as continuous color development without a change in fluorescent intensity. Optimization of the detection antibody or HRP conjugate is required to determine the linear range of a particular assay system.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What types of plates are compatible with the QuantaRed Enhanced Chemifluorescent HRP Substrate?

Black opaque plates typically produce the best results. White opaque plates also can be used, but might produce higher backgrounds. A variety of other plate types, such as gray or opaque plates with transparent bottoms are also useful with this substrate. Use clear plates if the absorbance of the colored product will be measure instead of the fluorescence.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.