TAT protein transduction into isolated perfused hearts: TAT-apoptosis repressor with caspase recruitment domain is cardioprotective.
Authors Gustafsson Asa B; Sayen M Richard; Williams Scott D; Crow Michael T; Gottlieb Roberta A;
JournalCirculation
PubMed ID12163436
'BACKGROUND: Linkage of the 11-amino-acid transduction domain of HIV TAT to a heterologous protein allows the protein to be transduced readily into cells. METHODS AND RESULTS: In this study, we inserted the apoptosis repressor with caspase recruitment domain (ARC) or beta-galactosidase (beta-gal) cDNA into the pTAT-hemagglutinin bacterial expression vector to ... More
Principles of 3' splice site selection and alternative splicing for an unusual group II intron from Bacillus anthracis.
AuthorsRobart AR, Montgomery NK, Smith KL, Zimmerly S,
JournalRNA
PubMed ID15100440
We investigated the self-splicing properties of two introns from the bacterium Bacillus anthracis. One intron (B.a.I1) splices poorly in vitro despite having typical structural motifs, while the second (B.a.I2) splices well while having apparently degenerated features. The spliced exons of B.a.I2 were sequenced, and splicing was found to occur at ... More