"Sal I -""DISCONTINUED""" - FAQs

Which restriction enzymes can exhibit star activity, and what factors contribute to star activity?

Star activity can be seen with many enzymes. See below for a list of some enzymes for which star activity has been reported. Some suggested causes for star activity include high glycerol concentrations, incorrect NaCl concentrations, and Mn2+ being substituted for Mg2+.

Some known enzymes that can exhibit star activity:
AdeI, AfaI, AloI, Alw26I, ApoI, AseI, AvaI *, AvaII, BamHI *, BanI, BanII, BceAI, BclI, BfmI, BfuI, BglI, Bme216I, BplI, Bpu10I, BseXI, Bsh1285I, Bsh1365I, BshTI, Bsp68I, Bsp143I, Bsp143II, BspMI, BsrBRI, BssHII, BstI, Bst1107I, BstEII, BstPI, BstXI, BsuRI, CeqI, CfrI, Cfr9I, Cfr10I, CviJI 2843, Eam1105I, Eco31I, Eco32I, Eco57I, Eco72I, Eco105I, EcoKMcrBC, EcoO65I, EcoRI *, M.EcoRI, EcoRV *, EcoT22I, EheI, FbaI, HaeIII *, HhaI *, HindIII *, HinfI *, HpaI *, HphI, KspAI, MamI, MbiI, MboII, MluI *, Mph1103I, MspA1I, MunI, MvaI, NciI, NcoI *, NcuI, NdeI *, NgoMIV, NheI *, PagI, PauI, PdmI, PmeI, PpiI, PshBI, PstI *, PsuI, PvuII *, RsrI, SacI, SalI*, Sau3AI, ScaI *, SchI, SdaI, SduI, SfiI, SgfI, SgrAI, SmaI *, SpeI *, Sse838, SspI *, SstI *, SwaI, TaqI *, TatI, Tth111I, TthHB8I, Van91I, XapI, XbaI *
* sold by Thermo Fisher Scientific