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Abstract
Hybrid selection of discrete genomic intervals on custom-designed microarrays for massively parallel sequencing.
AuthorsHodges E, Rooks M, Xuan Z, Bhattacharjee A, Benjamin Gordon D, Brizuela L, Richard McCombie W, Hannon GJ,
JournalNat Protoc
PubMed ID19478811
'Complementary techniques that deepen information content and minimize reagent costs are required to realize the full potential of massively parallel sequencing. Here, we describe a resequencing approach that directs focus to genomic regions of high interest by combining hybridization-based purification of multi-megabase regions with sequencing on the Illumina Genome Analyzer ... More