Bal I - FAQs

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2 个常见问题解答

What are the suggested conditions for restriction enzyme digestions?

Conditions can vary, so it is always best to follow any protocols provided with your specific enzyme. But the majority of enzymes to have similar protocols and here are some general guidelines:

- Most restriction enzyme digestions require 2-10 units enzyme/µg DNA.
- Restriction enzyme and the appropriate reaction buffer should be added to your DNA solution, and the volume of enzyme added should generally not be more than 10% of the DNA solution volume.
- Reaction buffers (like REact) are usually provided at 10X concentration, so they should be added such that their volume will represent exactly 1/10 of the final volume of DNA + restriction components.
- Recommended incubations are usually 1-2 hours at 37 degrees C.

I would like to perform a restriction enzyme double digestion. Do you offer a tool to determine optimal reaction conditions?

Please use our DoubleDigest Calculator linked below: https://www.thermofisher.com/us/en/home/brands/thermo-scientific/molecular-biology/thermo-scientific-restriction-modifying-enzymes/restriction-enzymes-thermo-scientific/double-digest-calculator-thermo-scientific.html