Trypsin-EDTA (0.5%), no phenol red - FAQs

View additional product information for Trypsin-EDTA (0.5%), no phenol red - FAQs (15400054)

18 product FAQs found

解冻后的胰酶可在冰箱中放置多长时间？

通常情况下，胰酶可在冰箱中保存一周。不过，这一时间长度也随着这一周内的加热次数和使用频率而变化。同时，它也与细胞粘附的紧密程度有关，胰酶在非冰冻条件下每天都会丧失一定的活性。

Gibco TrypLE试剂与胰酶相比优势在于？

TrypLE试剂不含动物源性与人源成份，纯度极高且对细胞的作用十分温和。该产品无需使用胰酶抑制剂对其进行终止。在含血清和不含血清的培养条件下，TrypLE试剂都是解离各类贴壁培养的哺乳动物细胞系的理想之选，能够直接做为胰酶替代品而应用于您当前的实验方案。

TrypLE试剂在常温下十分稳定，可随时按需取用。TrypLE细胞解离试剂能够在室温下稳定保存24个月，储存与操作都更为容易方便。

你们提供哪些用于解离细胞的试剂？这些产品之间的主要区别是什么？

请通过此选择列表(https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html)来比较我们的细胞解离试剂。

你们是否提供不含酚红的培养基（无酚红培养基）？

是的，请访问此页面(https://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf)浏览我们的无酚红培养基页面。

I received Trypsin-EDTA (0.5%), no phenol red (Cat. No. 15400054) with ice packs instead of dry ice and the product was partially melted. Is it OK to use?

Trypsin is shipped on wet ice as the shipment on dry ice can cause changes in pH which influences the product quality negatively. If you receive the trypsin thawed or partially thawed, the stability and functionality of the product will not be impacted. For further information please see the white paper at the following link.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the percentage of trypsin within the Trypsin-EDTA (0.5%), no phenol red (Cat. No. 15400054)?

Trypsin-EDTA (0.5%), no phenol red (Cat. No. 15400054 contains 0.5 % trypsin and 0.2% EDTA.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How do you recommend aliquoting Trypsin-EDTA (0.5%), no phenol red (Cat. No. 15400054)?

We recommend aliquoting the undiluted trypsin into single-use portions and storing the aliquots at -20 degrees C, so that fresh dilutions can be made whenever needed.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How many freeze/thaw cycles do you recommend for Trypsin-EDTA (0.5%), no phenol red (Cat. No. 15400054)?

We recommend keeping the freeze thaw cycles of trypsin to a minimum to avoid loss of enzyme activity.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What method do you recommend for thawing Trypsin-EDTA (0.5%), no phenol red (Cat. No. 15400054)?

We recommend thawing Trypsin-EDTA (0.5%) at 4 degrees C overnight. Alternatively, you can thaw it in a 37 degrees C water bath, but you will need to keep a close eye on it because extended exposure at that temperature can cause a decrease in enzyme activity. If you use the 37 degrees C thawing method, we recommend swirling the bottle every 5 mins to allow the heat to distribute evenly. As soon as the trypsin is thawed, remove it from the water bath.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What trypsin concentration should be used to dissociate cells?

The trypsin concentration required for dissociation varies with cell type and experimental requirements. See the complete range of Gibco Trypsin solutions and recommended dissociation conditions here (http://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/trypsin.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What solution is the 10X Trypsin EDTA in?

It is in 8.5g/L NaCl.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What can I dilute my 10X Trypsin EDTA with?

Concentrated forms of trypsin can be aseptically diluted to 1X using a balanced salt solution without calcium and magnesium (eg. HBSS,Cat. No. 14170120).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Does Trypsin EDTA 0.5% solution (Cat. No. 15400xxx) contain Phenol Red?

No, this Trypsin EDTA solution is without Phenol Red

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep trypsin in the refrigerator after thawing?

Generally speaking, trypsin can be stored refrigerated for up to a week. However, this may vary depending on how often it is warmed up and used during that week. It may also depend on how tightly the cells are attached, because there will be some loss of activity each day the trypsin is not frozen.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between TrypLE Express Enzyme, TrypLE Select Enzyme, and CTS TrypLE Select Enzyme?

All the above products contain rProtease, a non-animal, trypsin-like enzyme formulation used for the dissociation of attachment-dependent cell lines. This alternative for porcine trypsin is a recombinant enzyme derived from microbial fermentation.

The TrpLE Express, TrypLE Select, and CTS TrypLE Select products differ in their production and testing methods and in the documentation provided.

TrypLE Select is for the industrial market and has release-testing both for endotoxins and enzyme activity. This material also has a Drug Master File on record. It is manufactured in AOF-dedicated equipment and is intended for Research Use/Further Cell Culture Manufacturing.

TrypLE Express is manufactured without use of dedicated equipment and is intended for Research Use Only. Not for use in diagnostic procedures.

CTS TrypLE Select is manufactured under scalable cGMP conditions using dedicated equipment and is designed for clinical applications. This product has a Drug Master File on record and is intended for Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. Not intended for direct administration into humans or animals.

To read more about TrypLE and cell release times using this reagent, please go here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/tryple-express.html#1).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

What are the benefits of Gibco TrypLE reagent over trypsin?

TrypLE reagent is free of animal- and human-derived components, producing an exceptionally pure reagent that is gentle on cells. Inactivation with trypsin inhibitors is not required. TrypLE reagent is ideal for dissociating a variety of attachment-dependent mammalian cell lines both in serum and in serum-free conditions, and can be directly substituted for trypsin in your current protocol. TrypLE reagent is room-temperature stable and ready to use when you need it. TrypLE cell dissociation reagents remain stable for 24 months at room temperature, making storage and handling easier and more convenient.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

Do you offer media without phenol red (phenol red-free media)?

Yes, please visit this page for a list of our phenol red-free media (http://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.