AuthorsDe Gassart A, De Angelis Rigotti F, Gatti E,
JournalMethods Mol Biol
PubMed ID23329511
'Ubiquitinated protein detection is often troublesome since in most cases this modification reduces the half-life of targeted proteins, inducing their degradation. Furthermore, ubiquitination is reversible thanks to the action of highly specific deubiquitinases present in all eukaryotic cells. MHC molecules ubiquitination has been demonstrated to be a key event in ... More
Pressure cycling technology (PCT) reduces effects of inhibitors of the PCR.
AuthorsMarshall PL, King JL, Lawrence NP, Lazarev A, Gross VS, Budowle B,
JournalInt J Legal Med
PubMed ID22986474
A common problem in the analysis of forensic human DNA evidence, or for that matter any nucleic acid analysis, is the presence of contaminants or inhibitors. Contaminants may copurify with the DNA, inhibiting downstream PCR or they may present samples effectively as containing fewer templates than exist in the PCR, ... More
Expanding the diagnostic use of PCR in leptospirosis: improved method for DNA extraction from blood cultures.
AuthorsVillumsen S, Pedersen R, Krogfelt KA, Jensen JS,
JournalPLoS One
PubMed ID20711446
Leptospirosis is a neglected zoonosis of ubiquitous distribution. Symptoms are often non-specific and may range from flu-like symptoms to multi-organ failure. Diagnosis can only be made by specific diagnostic tests like serology and PCR. In non-endemic countries, leptospirosis is often not suspected before antibiotic treatment has been initiated and consequently, ... More
Production of compartmented cultures of rat sympathetic neurons.
AuthorsCampenot RB, Lund K, Mok SA,
JournalNat Protoc
PubMed ID20010935
The compartmented culture, in which primary neurons plated in a proximal compartment send their axons under silicone grease barriers and into left and right distal compartments, has enhanced the experimental capabilities of neuronal cultures. Treatments can be applied separately to cell bodies/proximal axons or distal axons, and cell bodies/proximal axons ... More