Multiple Infections of Ixodes scapularis Ticks by Borrelia bufdorferi as Revealed by Single-Strand Conformation Polymorphism Analysis
AuthorsGuttman DS, Wang PW, Wang IN, Bosler EM, Luft BJ, Dykhuizen DE
JournalJ Clin Micro
PubMed ID8904432
'The genetic heterogeneity of the spirochete Borrelia burgdorferi within single adult black-legged ticks from Shelter Island, N.Y., was determined by cold, single-strand conformation polymorphism (SSCP) analysis. The central region of the ospA gene of B. burgdorferi from infected ticks was amplified by nested PCR. Amplified product of the correct size ... More
Construction and characterization of a large-fragment chicken bacterial artificial chromosome library.
Authors Zimmer R; Verrinder Gibbins A M;
JournalGenomics
PubMed ID9192841
A large-insert bacterial artificial chromosome (BAC) library has been constructed from male chicken genomic DNA using the new pBeloBAC11 vector. The library was prepared in two parts, such that two-thirds of the BAC library (2976 clones) had an average insert size of 490 kb (80 clones analyzed), after optimization of ... More