Semiquantitative analysis of gene expression in cultured chondrocytes by RT-PCR.
AuthorsRolland-Valognes G,
JournalMethods Mol Med
PubMed ID15280588
'Reverse transcriptase-polymerase chain reaction (RT-PCR) is a powerful, sensitive, and rapid method to monitor small amounts of nucleic acids. This is of particular interest for small amounts of cells, as in cartilage. We present here two protocols to isolate total RNA and a protocol to study matrix metalloproteinase and type ... More
A pancoronavirus RT-PCR assay for detection of all known coronaviruses.
AuthorsVijgen L, Moës E, Keyaerts E, Li S, Van Ranst M,
JournalMethods Mol Biol
PubMed ID19057882
'The recent discoveries of novel human coronaviruses, including the coronavirus causing SARS, and the previously unrecognized human coronaviruses HCoV-NL63 and HCoV-HKU1, indicate that the family Coronaviridae harbors more members than was previously assumed. All human coronaviruses characterized at present are associated with respiratory illnesses, ranging from mild common colds to ... More
Isolating aptamers using capillary electrophoresis-SELEX (CE-SELEX).
AuthorsMosing RK, Bowser MT,
JournalMethods Mol Biol
PubMed ID19377982
SELEX (systematic evolution of ligands by exponential enrichment) is a process for isolating DNA or RNA sequences with high affinity and selectivity for molecular targets from random sequence libraries. These sequences are commonly referred to as aptamers. The process typically requires 10-15 cycles of enrichment, PCR amplification and nucleic acid ... More