HEPES (1M)
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HEPES (1M)
HEPES (1M)
HEPES (1M)
引用和文献 (6)
Gibco™

HEPES (1M)

Gibco™ HEPES（N-2-羟乙基哌嗪-N-2-乙烷磺酸）是一种两性离子有机化学缓冲剂，常用于细胞培养基中。当需要在 CO2 培养箱外长时间处理细胞培养物时，添加 10–25 mM HEPES了解更多信息
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货号数量
15630080100 mL
1563010620 mL
1563013020 x 100 mL
货号 15630080
价格（CNY)
1,464.00
Each
添加至购物车
数量:
100 mL
价格（CNY)
1,464.00
Each
添加至购物车
Gibco™ HEPES（N-2-羟乙基哌嗪-N-2-乙烷磺酸）是一种两性离子有机化学缓冲剂，常用于细胞培养基中。当需要在 CO2 培养箱外长时间处理细胞培养物时，添加 10–25 mM HEPES 可提供额外的缓冲能力。HEPES 具有膜不透性、对生化反应的影响有限、化学和酶学稳定性、极低的可见光和紫外光吸光度等优点，因此是许多细胞培养系统的良好缓冲选择。

两地 cGMP 生产和质量体系
Gibco™ HEPES 在 cGMP 合规机构生产，公司地址为英国苏格兰的 Paisley。该工厂是在 FDA 注册的医疗器械生产商，且通过 ISO 13485 标准认证。为确保供应链的稳定，我们同时提供由我们格兰德岛工厂生产的等同 Gibco™ HEPES 产品 (15630-080)。本工厂者亦是在 FDA 登记的医疗器械生产商，且符合 ISO 13485 标准。
仅用于研究和生产用途。不可用于临床诊断或直接用于人类或动物。
规格
化学名称或材料Zwitterionic Organic Chemical Buffer
颜色澄清溶液
配方N-2-Hydroxyethylpiperazine-N-2-Ethane Sulfonic Acid
建议的储存条件储存条件：2°C 至8°C
运输条件：室温
有效期：自生产之日起24个月
无菌Sterile
灭菌方法Sterile-filtered
适用于（应用）染色质生物学、哺乳动物细胞培养
分级生化
物理形态液体
数量100 mL
溶液类型Buffer
Unit SizeEach

常见问题解答 (FAQ)

Based on the Sodium bicarbonate levels in the medium, what CO2 % is recommended?

If the media formulation contains:

NaHCO3 (g/L) < 1.5, it needs CO2 at 4%;
NaHCO3 (g/L) 1.5 – 2.2, it needs CO2 at 5%;
NaHCO3 (g/L) 2.2 - 3.4, it needs CO2 at 7%;
NaHCO3 (g/L) > 3.5, it needs CO2 at 10% .

*There are some exceptions. Gibco DMEM has always been made according to Dulbecco’s original published formulation, with 3.7 g/L sodium bicarbonate. Customers have been using this medium in CO2 incubators ranging from 5-10% CO2 for decades, usually with no trouble maintaining physiological pH. This also depends on the cell type. Once cells are growing, the pH will drop (due to metabolic accumulation of lactic acid).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do high or low levels of sodium bicarbonate affect the pH of my tissue culture media?

Sodium bicarbonate is necessary to control the pH of the solution. To maintain physiological pH, the concentration of the sodium bicarbonate in the medium must be matched with the right level of CO2 in the atmosphere above the medium in the incubator*.

If the sodium bicarbonate is high and the CO2 concentration is low, the pH will become alkaline. This is something that you will see in a bottle of media when it is exposed to air for long periods or when there is a lot of head space in the bottle. The media color will be pinkish or purplish. When this media is put back into a CO2 incubator, the color/pH will change back to the normal orangeish/reddish color or physiological pH.

If the sodium bicarbonate is low and the CO2 is high, the pH will become acidic. This is something you will see when you put medium into a 5% or 10% CO2 incubator.

*There are some exceptions. For instance, Gibco DMEM has always been made according to Dulbecco’s original published formulation, with 3.7g/L sodium bicarbonate. Customers have been using this medium in CO2 incubators ranging from 5-10% CO2 for decades, usually with no trouble maintaining physiological pH. this also depends on the cell type, Once cells are growing, the pH will drop (due to metabolic accumulation of lactic acid).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My Gibco HEPES (1M) (Cat. No. 15630080) was accidentally frozen. Can I still use it?

This product should be stored at 2-8 degrees C and should not be frozen. The biggest issue with accidentally freezing this product is its solubility. If this product was accidentally frozen, we recommend placing it in a 2-8 degree environment and allowing it to slowly thaw overnight. If the product is fully thawed and shows no signs of precipitation, then it should still be usable, but we cannot guarantee effectiveness.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is HEPES (1 M) in distilled water or in saline solution?

1M HEPES is in water with a pH range of 7.2 - 7.5.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the endotoxin specification for HEPES (1 M) (Cat. Nos. 15630080, 15630106, and 15630130)?

Sorry, we do not test endotoxin levels for this product.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

引用和文献 (6)

引用和文献
Abstract
A comparison between different human hepatocyte models reveals profound differences in net glucose production, lipid composition and metabolism in vitro.
Authors:Bonanini F,Singh M,Yang H,Kurek D,Harms AC,Mardinoglu A,Hankemeier T
Journal:Experimental cell research
PubMed ID:38499143
Preparation and characterization of toxic Abeta aggregates for structural and functional studies in Alzheimer's disease research.
Authors:Jan A, Hartley DM, Lashuel HA,
Journal:Nat Protoc
PubMed ID:20539293
'The amyloid cascade hypothesis, supported by strong evidence from genetics, pathology and studies using animal models, implicates amyloid-beta (Abeta) oligomerization and fibrillogenesis as central causative events in the pathogenesis of Alzheimer''s disease (AD). Today, significant efforts in academia, biotechnology and the pharmaceutical industry are devoted to identifying the mechanisms by ... More
Investigating conversion of mechanical force into biochemical signaling in three-dimensional chondrocyte cultures.
Authors:Bougault C, Paumier A, Aubert-Foucher E, Mallein-Gerin F,
Journal:Nat Protoc
PubMed ID:19478808
The culture of chondrocytes embedded within agarose hydrogels maintains chondrocytic phenotype over extended periods and allows analysis of the chondrocyte response to mechanical forces. The mechanisms involved in the transduction of a mechanical stimulus to a physiological process are not completely deciphered. We present protocols to prepare and characterize constructs ... More
Molecular rearrangements of the extracellular vestibule in NMDAR channels during gating.
Authors: Sobolevsky Alexander I; Beck Christine; Wollmuth Lonnie P;
Journal:Neuron
PubMed ID:11779481
Many N-methyl-D-aspartate receptor (NMDAR) channel blockers that have therapeutic potential can be trapped in the closed state. Using a combination of the substituted cysteine accessibility method and open channel blockers, we found that the M3 segment forms the core of the extracellular vestibule, including a deep site for trapping blockers. ... More
Polycystin-1, the product of the polycystic kidney disease 1 gene, co-localizes with desmosomes in MDCK cells.
Authors:Scheffers MS, van der Bent P, Prins F, Spruit L, Breuning MH, Litvinov SV, de Heer E, Peters DJ,
Journal:Hum Mol Genet
PubMed ID:11063733
Polycystin-1 is a novel protein predicted to be a large membrane-spanning glycoprotein with an extracellular N-terminus and an intracellular C-terminus, harboring several structural motifs. To study the subcellular localization, antibodies raised against various domains of polycystin-1 and against specific adhesion complex proteins were used for two-color immunofluorescence staining. In Madine ... More
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