Involvement of c-Src Tyrosine Kinase Upstream of Class I Phosphatidylinositol (PI) 3-Kinases in Salmonella Enteritidis Rck Protein-mediated Invasion.
AuthorsWiedemann A, Rosselin M, Mijouin L, Bottreau E, Velge P,
JournalJ Biol Chem
PubMed ID22810232
'The Salmonella outer membrane protein Rck mediates a Zipper entry mechanism controlled by tyrosine phosphorylation and class I phosphatidylinositol 3-kinase (PI 3-kinase). However, the underlying mechanism leading to this signaling cascade remains unclear. The present study showed that using Rck-coated beads or Rck-overexpressing Escherichia coli, Rck-mediated actin polymerization and invasion ... More
Isolation of carbohydrate-specific CD4(+) T cell clones from mice after stimulation by two model glycoconjugate vaccines.
AuthorsAvci FY, Li X, Tsuji M, Kasper DL,
JournalNat Protoc
PubMed ID23196974
Here we describe how to isolate carbohydrate-specific T cell clones (for which we propose the designation 'Tcarbs') after stimulation by two glycoconjugate vaccines. We describe how to prepare, purify and characterize two model glycoconjugate vaccines that can be used to generate Tcarbs. These glycoconjugate vaccines (GBSIII-OVA and GBSIII-TT) are synthesized ... More
A three-dimensional co-culture system to investigate macrophage-dependent tumor cell invasion.
AuthorsDwyer AR, Ellies LG, Holme AL, Pixley FJ
JournalJ Biol Methods
PubMed ID31453214
'Macrophages infiltrate cancers and promote progression to invasion and metastasis. To directly examine tumor-associated macrophages (TAMs) and tumor cells interacting and co-migrating in a three-dimensional (3D) environment, we have developed a co-culture model that uses a PyVmT mouse mammary tumor-derived cell line and mouse bone marrow-derived macrophages (BMM). The Py8119 ... More
Characterizing the Antimicrobial Function of a Dairy-Originated Probiotic,
AuthorsNair DVT, Kollanoor Johny A
JournalFront Microbiol
PubMed ID30050507
'Antimicrobial potential of a dairy-origin probiotic bacteria,'
Palbociclib treatment alters nucleotide biosynthesis and glutamine dependency in A549 cells.
AuthorsConroy LR, Lorkiewicz P, He L, Yin X, Zhang X, Rai SN, Clem BF
JournalCancer Cell Int
PubMed ID32624705
Aberrant activity of cell cycle proteins is one of the key somatic events in non-small cell lung cancer (NSCLC) pathogenesis. In most NSCLC cases, the retinoblastoma protein tumor suppressor (RB) becomes inactivated via constitutive phosphorylation by cyclin dependent kinase (CDK) 4/6, leading to uncontrolled cell proliferation. Palbociclib, a small molecule ... More