pTK-Red Firefly Luc Vector for Luciferase Assays - FAQs

Q: What is the stability of the secreted luciferase?

Gaussia, Gaussia -Dura , and Cypridina Luciferase are stable in the culture media for greater than 24 hours. Generally, after transfection of CMV driven constructs, we see ever increasing secreted signal between 24 and 72 hours. Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center .

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4 个常见问题解答

What options do you offer for luciferase vectors?

There are three vectors available for each expressed luciferase; pMCS, pCMV, and pTK. We recommend using pMCS to measure the activity of the reporter, and pCMV, and pTK as positive control vectors. For high level constitutive expression of Luciferase, we recommend using the CMV (Cytomegalovirus) promoter vector and for low level constitutive expression of luciferase, we recommend using the pTK (thymidine kinase) promoter vector.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the stability of the secreted luciferase?

Gaussia, Gaussia-Dura, and Cypridina Luciferase are stable in the culture media for greater than 24 hours. Generally, after transfection of CMV driven constructs, we see ever increasing secreted signal between 24 and 72 hours.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How quickly is luciferase secreted?

The earliest detectable signal will depend on the strength of the promoter. In general, with a strong promoter (such as CMV), significant signal over background can be seen in as little as 20 minutes. However, with a weaker inducible promoter, significant signal over background may take 1-2 hours after induction.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do you still offer quantitative IP (qIP) Luciferase assay kits?

Our qIP Luciferase assay kits have been discontinued but we do carry epitope-tagged (HA or c-Myc) and Tluc (TurboLuc luciferase)-tagged mammalian expression vectors, and qIP protein interaction assay reagents for these assay kits.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.