Pierce™ Gaussia-萤火虫荧光素酶双检测试剂盒
Pierce™ Gaussia-萤火虫荧光素酶双检测试剂盒
Thermo Scientific™

Pierce™ Gaussia-萤火虫荧光素酶双检测试剂盒

Thermo Scientific Pierce Gaussia-萤火虫荧光素酶双检测试剂盒提供了同时检测哺乳动物全细胞裂解物中的细胞内 Gaussia 和红色萤火虫荧光素酶活的必要试剂性。Gaussia-萤火虫荧光素酶双检测试剂盒的特点：•同时—对两种荧光素酶活性同时进行基于过滤器的波长分离检测• 灵敏—测量同一样品中的亮蓝色 Gaussia 和红色萤火虫荧光素酶活性•了解更多信息
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货号数量
16181100 次反应试剂盒
161821000 次反应试剂盒
货号 16181
价格（CNY)
1,167.00
飞享价
Ends: 31-Dec-2025
1,572.00
共减 405.00 (26%)
Each
添加至购物车
数量:
100 次反应试剂盒
价格（CNY)
1,167.00
飞享价
Ends: 31-Dec-2025
1,572.00
共减 405.00 (26%)
Each
添加至购物车
Thermo Scientific Pierce Gaussia-萤火虫荧光素酶双检测试剂盒提供了同时检测哺乳动物全细胞裂解物中的细胞内 Gaussia 和红色萤火虫荧光素酶活的必要试剂性。

Gaussia-萤火虫荧光素酶双检测试剂盒的特点：

•同时—对两种荧光素酶活性同时进行基于过滤器的波长分离检测
• 灵敏—测量同一样品中的亮蓝色 Gaussia 和红色萤火虫荧光素酶活性
• 快速—与传统的序贯双测定不同，无需淬灭步骤
• 多重分析—可以对同一样品中的两种细胞活性进行定量

Pierce Gaussia-萤火虫荧光素酶双检测试剂盒是一种高灵敏度的测定，支持同时检测 Gaussia 和红色萤火虫荧光素酶活性。Gaussia 荧光素酶充当一个实验报告基因，以组成型活化的红色萤火虫作为一种归一化对照。如以下动画视频所示，该报告基因对照组合可在单次读码测定中同时监测实验报告基因和对照荧光素酶的活性，无需双步骤添加底物试剂或淬灭。该测定工作溶液包含两种荧光素酶的底物，且在反应的同时出现闪光型动力学。所产生的发光信号可以使用过滤器进行光谱解析。在单个样品中，研究人员可以测定调节元素的转录活性、信号转导通路以及活化剂或抑制剂的影响。

产品组分：
细胞裂解缓冲液、反应缓冲液以及底物 Vargulin 和 D-萤光素

需要：
海萤和红色萤火虫荧光素酶报告基因；滤光片组的范围为 425-525nm（对于海萤 Luc）和 615-675nm（对于红色萤火虫 Luc）。光度计或其他能够监测发光的仪器（如 Thermo Scientific Luminoskan Ascent 和 Varioskan 闪光酶标仪）；一次评估超过 24 孔所需的进样器。

应用：
•同时研究两种调节元素
• 同时监测两条信号通路
• 每次筛选可研究多个靶标（例如脱靶效应）

应用视频：
Gaussia 荧光素酶可产生比萤火虫和天然海肾荧光素酶更明亮的生物发光信号。Gaussia 荧光素酶产生的生物发光信号 (λmax= 470nm) 是由腔肠素氧化引起的。该反应不需要腺苷三磷酸 (ATP) 或其他辅因子。红色萤火虫荧光素酶是来源于源氏萤的日本源氏萤火虫荧光素酶的突变形式。该荧光素酶产生了一种红移辐射光（λ最大 = 613nm），它是由 D-荧光素在 ATP 存在的情况下氧化引起的。红色萤火虫和 Gaussia 荧光素酶用于双测定，因为光输出可进行光谱解析。

更多产品数据
•极灵敏的多通路荧光素酶报告基因测定
•荧光素酶报告基因测定的同步双发射检测
•通过农药化学品激活抗氧化反应途径
仅供科研使用。不可用于诊断程序。
规格
检测报告基因酶、荧光素酶报告基因检测
适用细胞哺乳动物细胞
检测方法生物发光
适用于（设备）光度计（微孔板）
产品规格384 孔板、96 孔板
标签类型酶标记
产品线Pierce™
数量100 次反应试剂盒
底物腔肠素、D-荧光素
底物属性化学底物
底物类型荧光素酶底物
靶标荧光素酶、萤火虫荧光素酶、Gaussia 荧光素酶
技术增强的化学发光法
类型检测试剂盒
Unit SizeEach

常见问题解答 (FAQ)

I got a high background signal when I used a Pierce Gaussia/Cypridina/Renilla -Firefly Dual Assay Kit. Why is this?

Here are possible causes and solutions:

- Non-specific oxidation of substrate: Use a new sample; Avoid repeated freezing and thawing of the sample.
- Control sample is contaminated: Change pipette tips after each well; Reduce shaker speed during the cell lysis step to avoid contaminating the wells.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I got a high signal when I used a Pierce Gaussia/Cypridina/Renilla -Firefly Dual Assay Kit. Why is this?

This could be due to high luciferase expression. Here are some suggestions:

  • Reduce incubation time before collecting samples. 
  • Decrease the integration time on the instrument.
  • Dilute the sample: 
    Note: A low sample volume can increase assay variability. Dilute the sample and use the recommended volume of 10-20 µL per assay.



    • Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

    I got a low signal in my lysate when I used a Pierce Gaussia/Cypridina/Renilla-Firefly Dual Assay Kit. Why is this?

    Here are possible causes and solutions:

    - Low luciferase expression: Lyse cells in smaller volume of 1X Cell Lysis Buffer; Use a different promoter or growth conditions to improve expression; Increase the integration time on the instrument; Scale up volume of sample and reagent per well.

    Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

    I did not get any signal when I used a Pierce Gaussia/Cypridina/Renilla-Firefly Dual Assay Kit. Why is this?

    Here are possible causes and solutions:

    - Low transfection efficiency: Optimize transfection conditions using a visual transfection control (e.g., a plasmid over-expressing a fluorescent protein); Verify plasmid DNA quality using restriction digestion and agarose gel electrophoresis; Use only transfection grade DNA - please note that most high-quality plasmid DNA should be supercoiled; Use actively dividing, low-passage cells; Use a different cell type.
    - No promoter induction: Incubate cells using promoter-specific inducing conditions; Incubate cells for a longer time after treatment; Change growth conditions to improve expression; Use a different promoter.
    - Substrate auto-oxidized: Protect substrate from light and air; Maintain 100X Coelenterazine at -80 degrees C, 100X Vargulin at -20 degrees C, and 100X D-Luciferin at -20 degrees C.

    Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

    I got a high background signal with a Gaussia/Cypridina/Renilla Luciferase Glow/Luciferase Flash assay. What went wrong?

    Here are possible causes and solutions:

    - Non-specific oxidation of substrate: Use less serum in the cell culture media; Note: Albumin can increase the auto-oxidation of Vargulin; Avoid repeated freezing and thawing of the sample.
    - Control sample is contaminated: Use new sample; Change pipette tips after each well.

    Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

    View all Pierce™ Gaussia-萤火虫荧光素酶双检测试剂盒 FAQs