Pierce™ 海肾-萤火虫荧光素酶双检测试剂盒
Pierce™ 海肾-萤火虫荧光素酶双检测试剂盒
Thermo Scientific™

Pierce™ 海肾-萤火虫荧光素酶双检测试剂盒

Thermo Scientific Pierce 海肾-萤火虫荧光素酶双检测试剂盒提供了同时检测哺乳动物全细胞裂解物中的细胞内海肾和红色萤火虫荧光素酶活的必要试剂性。海肾-萤火虫荧光素酶双检测试剂盒的特点:•同时—对两种荧光素酶活性同时进行基于过滤器的波长分离检测• 灵敏—测量同一样品中的绿色海肾和红色萤火虫荧光素酶活性• 快速—与传统的序贯双测定不同,无需淬灭步骤• 多重分析—可以对同一样品中的两种细胞活性进行定量Pierce 海肾-萤火虫荧光素酶双检测试剂盒是一种高灵敏度的测定,支持同时检测海肾和红色萤火虫荧光素酶活性。海肾荧光素酶充当一个实验报告基因,以组成型活化的红色萤火虫作为一种归一化对照了解更多信息
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货号数量
161861000 次反应试剂盒
16185100 次反应试剂盒
货号 16186
价格(CNY)
10,727.00
Each
添加至购物车
数量:
1000 次反应试剂盒
价格(CNY)
10,727.00
Each
添加至购物车
Thermo Scientific Pierce 海肾-萤火虫荧光素酶双检测试剂盒提供了同时检测哺乳动物全细胞裂解物中的细胞内海肾和红色萤火虫荧光素酶活的必要试剂性。

海肾-萤火虫荧光素酶双检测试剂盒的特点:

同时—对两种荧光素酶活性同时进行基于过滤器的波长分离检测
灵敏—测量同一样品中的绿色海肾和红色萤火虫荧光素酶活性
快速—与传统的序贯双测定不同,无需淬灭步骤
多重分析—可以对同一样品中的两种细胞活性进行定量

Pierce 海肾-萤火虫荧光素酶双检测试剂盒是一种高灵敏度的测定,支持同时检测海肾和红色萤火虫荧光素酶活性。海肾荧光素酶充当一个实验报告基因,以组成型活化的红色萤火虫作为一种归一化对照。如以下动画视频所示,该报告基因对照组合可在单次读码测定中同时监测实验报告基因和对照荧光素酶的活性,无需双步骤添加底物试剂或淬灭。该测定工作溶液包含两种荧光素酶的底物,且在反应的同时出现闪光型动力学。所产生的发光信号可以使用过滤器进行光谱解析。在单个样品中,研究人员可以测定调节元素的转录活性、信号转导通路以及活化剂或抑制剂的影响。

包括:
细胞裂解缓冲液、反应缓冲液以及底物腔肠素和 D-萤光素

需要:
绿色海肾和红色萤火虫荧光素酶报告基因;滤光片组的波长为 525±20nm(对于绿色海肾 Luc)和 615 至 675nm(对于红色萤火虫 Luc)。光度计或其他能够监测发光的仪器(如 Thermo Scientific Luminoskan Ascent 和 Varioskan 闪光微孔板读数仪);一次评估超过 24 孔所需的进样器。

应用:
•同时研究两种调节元素
• 同时监测两条信号通路
• 每次筛选可研究多个靶标(例如脱靶效应)

绿色海肾荧光素酶可产生比萤火虫和天然海肾荧光素酶更明亮的生物发光信号。绿色海肾荧光素酶蛋白产生的生物发光信号 (λmax= 535nm) 是由腔肠素氧化引起的。第二项测定使用红色萤火虫荧光素酶是日本源氏萤火虫荧光素酶源氏萤的突变形式。该荧光素酶产生了一种红移辐射光谱 (λmax= 613nm),它是由 D-荧光素在 ATP 存在的情况下氧化引起的。

更多产品数据
荧光素酶报告基因测定的同步双发射检测

相关产品
Pierce™ 海肾萤火虫荧光素酶双检测试剂盒
仅供科研使用。不可用于诊断程序。
规格
检测报告基因酶、荧光素酶报告基因检测
适用细胞哺乳动物细胞
检测方法生物发光
适用于(设备)化学发光酶标仪(微孔板)
产品规格384 孔板、96 孔板
标签类型酶标记
产品线Pierce™
数量1000 次反应试剂盒
底物腔肠素、D-荧光素
底物属性化学底物
底物类型荧光素酶底物
足够用于1000 次双荧光素酶检测
靶标荧光素酶、萤火虫荧光素酶、绿色海肾荧光素酶
技术增强的化学发光法
类型检测试剂盒
Unit SizeEach

常见问题解答 (FAQ)

What are the advantages of using TurboFect Transfection Reagent (Cat. No. R0533) over other transfection reagents with the Pierce Renilla-Firefly Luciferase Dual Assay Kit (Cat. Nos. 16185, 16186)?

The Pierce Renilla-Firefly Luciferase Dual Assay Kit (Cat. Nos. 16185, 16186) luciferase vectors were optimized with TurboFect. However, it should be feasible to replace TurboFect with another transfection reagent if your cells tolerate the transfection reagent better and give you acceptable transfection rates.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I store my Pierce Renilla-Firefly Luciferase Dual Assay Kit (Cat. Nos. 16185, 16186) cell lysates at -20 degrees C to test later?

The stability of the Pierce Renilla-Firefly Luciferase Dual Assay Kit (Cat. Nos. 16185, 16186) may be inconsistent from sample to sample at -20 degrees C. We recommend snap-freezing lysates at -80 degrees C with protease inhibitors to help prevent protein degradation.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the Pierce Renilla-Firefly Luciferase Dual Assay Kit (Cat. Nos. 16185, 16186) with a luminometer that is not equipped with reagent injectors?

No. All Luciferase Dual Assay Kits, such as the Pierce Renilla-Firefly Luciferase Dual Assay Kit (Cat. Nos. 16185, 16186), are flash-based assays. Flash luminescence reactions give a very bright signal over a short reaction time. Luciferase assays that are flash-based require the use of a luminometer or other luminescence-monitoring instrument equipped with automated reagent injectors.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I got a high background signal when I used a Pierce Gaussia/Cypridina/Renilla -Firefly Dual Assay Kit. Why is this?

Here are possible causes and solutions:

- Non-specific oxidation of substrate: Use a new sample; Avoid repeated freezing and thawing of the sample.
- Control sample is contaminated: Change pipette tips after each well; Reduce shaker speed during the cell lysis step to avoid contaminating the wells.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I got a high signal when I used a Pierce Gaussia/Cypridina/Renilla -Firefly Dual Assay Kit. Why is this?

This could be due to high luciferase expression. Here are some suggestions:

  • Reduce incubation time before collecting samples. 
  • Decrease the integration time on the instrument.
  • Dilute the sample: 
    Note: A low sample volume can increase assay variability. Dilute the sample and use the recommended volume of 10-20 µL per assay.



  • Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.