FreeStyle™ MAX 试剂是一种专有的非动物源性制剂,用于将质粒 DNA 转染到真核细胞中,其可轻松扩大以生产大量重组蛋白。FreeStyle™ MAX 试剂可在生物生产应用中获得极高表达水平和转染率,细胞毒性较低,并经过特殊配制可与以下产品配合使用:•无血清 FreeStyle™ 293了解更多信息
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货号
数量
16447500
15 mL
16447100
1 mL
16447750
10 x 15 mL
3 选项
货号 16447500
价格(CNY)
184,221.00
Each
添加至购物车
数量:
15 mL
价格(CNY)
184,221.00
Each
添加至购物车
FreeStyle™ MAX 试剂是一种专有的非动物源性制剂,用于将质粒 DNA 转染到真核细胞中,其可轻松扩大以生产大量重组蛋白。FreeStyle™ MAX 试剂可在生物生产应用中获得极高表达水平和转染率,细胞毒性较低,并经过特殊配制可与以下产品配合使用:
•无血清 FreeStyle™ 293 表达培养基(货号 12338-018)中的 FreeStyle™ 293-F 细胞(悬浮人胚肾细胞,货号 R790-07) •无血清 FreeStyle™ CHO 表达培养基(货号 12651-014)中的 FreeStyle™ CHO-S 细胞(悬浮中国仓鼠卵巢细胞,货号 R800-07) •含 8 mM L-谷氨酰胺和 18 mL/L 10% Pluronic™ F-68 的 CD DG44 培养基(货号 A11000-01)中的 DG44 细胞(DHFR– 悬浮 CHO 细胞)
产品用途 FreeStyle™ MAX 试剂预期用于与 FreeStyle™ MAX 293 表达系统(货号 K9000-10)和 FreeStyle™ MAX CHO 表达系统(货号 K9000-20)配合使用。
仅供科研使用。不可用于诊断程序。
规格
分类无动物来源
适用于(应用)转染
高通量能力不兼容高通量应用(手动)
产品线Freestyle™
产品类型转染试剂
数量15 mL
血清兼容性是
运输条件湿冰
细胞类型已建立的细胞系
样品类型质粒 DNA
转染技术脂质体介导转染
Unit SizeEach
内容与储存
每单位包含一小瓶试剂。储存在 +4°C 下。妥善储存时,保证 FreeStyle™ MAX 稳定储存 6 个月。
What is the advantage of FreeStyle Max Reagent over 293fectin Transfection Reagent?
Both FreeStyle Max Reagent and 293fectin Transfection Reagent provide similar levels of high transfection efficiency; however, FreeStyle Max Reagent has lower cytotoxicity and hence results in higher protein yields. Additionally, FreeStyle Max Reagent is animal-origin free.
Can FreeStyle 293-F cells grown in FreeStyle 293 expression medium be transfected with ExpiFectamine293 Reagent along with enhancers? Is so, will the transfection efficiency be higher than that with FreeStyle Max Reagent?
You should be able to use the ExpiFectamine293 Reagent for transfection of Freestyle 293-F cells grown in FreeStyle 293 Expression Medium; however, the enhancers will provide only a little boost in expression as they are designed to work with higher density cultures that FreeStyle 293 Expression Medium cannot support.
Can FreeStyle MAX reagent be used for transfecting adherent cells?
Yes, the reagent can be used for adherent cells, however the protocol supplied with the reagent has been optimized for CHO and 293 cells in suspension culture. If you want to transfect adherent cells, please use Lipofectamine 2000 transfection reagent.