Proteomic analysis reveals presence of platelet microparticles in endothelial progenitor cell cultures.
AuthorsProkopi M, Pula G, Mayr U, Devue C, Gallagher J, Xiao Q, Boulanger CM, Westwood N, Urbich C, Willeit J, Steiner M, Breuss J, Xu Q, Kiechl S, Mayr M,
JournalBlood
PubMed ID19369228
'The concept of endothelial progenitor cells (EPCs) has attracted considerable interest in cardiovascular research, but despite a decade of research there are still no specific markers for EPCs and results from clinical trials remain controversial. Using liquid chromatography-tandem mass spectrometry, we analyzed the protein composition of microparticles (MPs) originating from ... More
A phantom for diffusion-weighted MRI (DW-MRI).
AuthorsLavdas I, Behan KC, Papadaki A, McRobbie DW, Aboagye EO,
JournalJ Magn Reson Imaging
PubMed ID23576443
'PURPOSE: To develop tissue-equivalent diffusivity materials and build a spherical diffusion phantom which mimics the conditions typically found in biological tissues. Also, to assess the reproducibility of ADC measurements from a whole-body diffusion protocol. MATERIALS AND METHODS: Nickel-doped agarose/sucrose gels were manufactured and used to build a spherical diffusion phantom ... More
Determinants of nucleosome organization in primary human cells.
AuthorsValouev A, Johnson SM, Boyd SD, Smith CL, Fire AZ, Sidow A,
JournalNature
PubMed ID21602827
'Nucleosomes are the basic packaging units of chromatin, modulating accessibility of regulatory proteins to DNA and thus influencing eukaryotic gene regulation. Elaborate chromatin remodelling mechanisms have evolved that govern nucleosome organization at promoters, regulatory elements, and other functional regions in the genome. Analyses of chromatin landscape have uncovered a variety ... More
Controlling cell position in complex heterotypic 3D microtissues by tissue fusion.
AuthorsRago AP, Dean DM, Morgan JR,
JournalBiotechnol Bioeng
PubMed ID19012266
'Tissue fusion and cell sorting are processes fundamental to developmental biology with applications in tissue engineering. We have designed a fusion assay to investigate the factors governing the fusion of microtissues and the cell sorting that occurs after fusion. Normal human fibroblast (NHF) spheroids were self-assembled and cultured for 1, ... More
Host alternation of chikungunya virus increases fitness while restricting population diversity and adaptability to novel selective pressures.
AuthorsCoffey LL, Vignuzzi M,
JournalJ Virol
PubMed ID21047966
'The mechanisms by which RNA arboviruses, including chikungunya virus (CHIKV), evolve and maintain the ability to infect vertebrate and invertebrate hosts are poorly understood. To understand how host specificity shapes arbovirus populations, we studied CHIKV populations passaged alternately between invertebrate and vertebrate cells (invertebrate ? vertebrate) to simulate natural alternation ... More
Circulating monocytes expressing CD31: implications for acute and chronic angiogenesis.
AuthorsKim SJ, Kim JS, Papadopoulos J, Wook Kim S, Maya M, Zhang F, He J, Fan D, Langley R, Fidler IJ,
JournalAm J Pathol
PubMed ID19349357
'To identify the roles of various circulating cells (eg, endothelial and/or stem and progenitor cells) in angiogenesis, we parabiosed a wild-type syngeneic mouse with a transgenic syngeneic green fluorescent protein mouse. Following the establishment of a common circulation between these parabionts, we investigated acute (7 to 10 days), subacute (2 ... More
Host cell-free growth of the Q fever bacterium Coxiella burnetii.
AuthorsOmsland A, Cockrell DC, Howe D, Fischer ER, Virtaneva K, Sturdevant DE, Porcella SF, Heinzen RA,
JournalProc Natl Acad Sci U S A
PubMed ID19246385
'The inability to propagate obligate intracellular pathogens under axenic (host cell-free) culture conditions imposes severe experimental constraints that have negatively impacted progress in understanding pathogen virulence and disease mechanisms. Coxiella burnetii, the causative agent of human Q (Query) fever, is an obligate intracellular bacterial pathogen that replicates exclusively in an ... More
DNase-seq: a high-resolution technique for mapping active gene regulatory elements across the genome from mammalian cells.
AuthorsSong L, Crawford GE,
JournalCold Spring Harb Protoc
PubMed ID20150147
Stress clamp experiments on multicellular tumor spheroids.
The precise role of the microenvironment on tumor growth is poorly understood. Whereas the tumor is in constant competition with the surrounding tissue, little is known about the mechanics of this interaction. Using a novel experimental procedure, we study quantitatively the effect of an applied mechanical stress on the long-term ... More
Molecular characterization of the complete genome of a street rabies virus isolated in China.
AuthorsMing P, Du J, Tang Q, Yan J, Nadin-Davis SA, Li H, Tao X, Huang Y, Hu R, Liang G,
JournalVirus Res
PubMed ID19463716
In this study, the complete genomic sequence of a rabies virus isolate HN10, recovered from brain tissue of a rabid patient in China, was determined. This is the first Chinese street isolate that has been fully characterized. The overall organization of this virus is typical of that observed for all ... More
Single copies of mutant KRAS and mutant PIK3CA cooperate in immortalized human epithelial cells to induce tumor formation.
AuthorsWang GM, Wong HY, Konishi H, Blair BG, Abukhdeir A, Gustin JP, Rosen DM, Denmeade S, Rasheed Z, Matsui W, Garay JP, Mohseni M, Higgins MJ, Cidado J, Jelovac D, Croessmann S, Cochran R, Karnan S, Konishi Y, Ota A, Hosokawa Y, Argani P, Lauring J, Park BH,
JournalCancer Res
PubMed ID23580570
The selective pressures leading to cancers with mutations in both KRAS and PIK3CA are unclear. Here we demonstrate that somatic cell knock in of both KRAS G12V and oncogenic PIK3CA mutations in human breast epithelial cells results in cooperative activation of the PI3 Kinase and MAP Kinase pathways in vitro, ... More
Concurrent blood-brain barrier opening and local drug delivery using drug-carrying microbubbles and focused ultrasound for brain glioma treatment.
AuthorsTing CY, Fan CH, Liu HL, Huang CY, Hsieh HY, Yen TC, Wei KC, Yeh CK,
JournalBiomaterials
PubMed ID22019122
Glioblastoma multiforme (GBM) is a highly malignant brain tumor. The blood-brain barrier (BBB) provides a major obstacle to chemotherapy since therapeutic doses cannot be achieved by traditional drug delivery without severe systemic cytotoxic effects. Recently, microbubble (MB)-enhanced focused ultrasound (FUS) was shown to temporally and locally disrupt the BBB thereby ... More
Bcl-2 family proteins contribute to apoptotic resistance in lung cancer multicellular spheroids.
AuthorsYang TM, Barbone D, Fennell DA, Broaddus VC,
JournalAm J Respir Cell Mol Biol
PubMed ID19097992
Combinatorial therapies using the proteasome inhibitor, bortezomib, have been found to induce synergistic apoptosis in cancer cells grown as monolayers; however, three-dimensional spheroid culture may be a better model for the multicellular resistance found in solid tumors, such as lung cancer. We tested the combinatorial apoptotic strategy of using bortezomib ... More
Comparison of two multilocus variable-number tandem-repeat methods and pulsed-field gel electrophoresis for differentiating highly clonal methicillin-resistant Staphylococcus aureus isolates.
In the United Kingdom, EMRSA-15 and EMRSA-16 account for the majority (~90%) of nosocomial methicillin-resistant Staphylococcus aureus (MRSA) infections. Currently, the standard typing technique, pulsed-field gel electrophoresis (PFGE), is laborious and insufficient for discriminating between closely related subtypes of EMRSA-15 and -16. The objective of the present study was to ... More
Direct contribution of inducible nitric oxide synthase expression to apoptosis induction in primary smooth chorion trophoblast cells of human fetal membrane tissues.
AuthorsYuan B, Ohyama K, Takeichi M, Toyoda H,
JournalInt J Biochem Cell Biol
PubMed ID18984062
We have previously demonstrated that apoptosis induction is observed only in smooth chorion laeve trophoblast cells, and not in amnion epithelial cells of human fetal membrane tissues prepared at the term. Apoptosis induction was suppressed by the presence of an inhibitor specific for inducible nitric oxide synthase (iNOS), suggesting that ... More
Prevalence of Mycobacterium avium subsp. paratuberculosis in ileocecal lymph nodes and on hides and carcasses from cull cows and fed cattle at commercial beef processing plants in the United States.
AuthorsWells JE, Bosilevac JM, Kalchayanand N, Arthur TM, Shackelford SD, Wheeler TL, Koohmaraie M,
JournalJ Food Prot
PubMed ID19681269
Clinical associations between Crohn's disease in humans and Mycobacterium avium subsp. paratuberculosis (MAP) have been suggested but not confirmed. Cattle could be sources for MAP, but little information on MAP prevalence with beef has been reported. Samples of ileocecal lymph nodes and swabs of hides and carcasses from 343 animals ... More
CUB-domain-containing protein 1 regulates peritoneal dissemination of gastric scirrhous carcinoma.
AuthorsUekita T, Tanaka M, Takigahira M, Miyazawa Y, Nakanishi Y, Kanai Y, Yanagihara K, Sakai R,
JournalAm J Pathol
PubMed ID18467693
CUB-domain-containing protein 1 (CDCP1) is a type-I transmembrane protein that is highly expressed in colon, breast, and lung cancers. We recently revealed that CDCP1 is associated with and phosphorylated by Src family kinases and is involved in the regulation of anchorage independence of certain lung cancer cell lines. In this ... More
Prevalence of diarrhoeagenic Escherichia coli in children from León, Nicaragua.
AuthorsVilchez S, Reyes D, Paniagua M, Bucardo F, Möllby R, Weintraub A,
JournalJ Med Microbiol
PubMed ID19369525
Diarrhoeal disease is a public health problem worldwide, mostly affecting children in developing countries. In Nicaragua, diarrhoea is the second greatest cause of infant mortality. During the period March 2005 to September 2006, a total of 526 faecal samples from children aged 0-60 months (381 with and 145 without diarrhoea) ... More
Knock-in of mutant K-ras in nontumorigenic human epithelial cells as a new model for studying K-ras mediated transformation.
AuthorsKonishi H, Karakas B, Abukhdeir AM, Lauring J, Gustin JP, Garay JP, Konishi Y, Gallmeier E, Bachman KE, Park BH,
JournalCancer Res
PubMed ID17875684
The oncogenic function of mutant ras in mammalian cells has been extensively investigated using multiple human and animal models. These systems include overexpression of exogenous mutant ras transgenes, conditionally expressed knock-in mouse models, and somatic cell knockout of mutant and wild-type ras genes in human cancer cell lines. However, phenotypic ... More
Pet dogs and chicken meat as reservoirs of Campylobacter spp. in Barbados.
AuthorsWorkman SN, Mathison GE, Lavoie MC,
JournalJ Clin Microbiol
PubMed ID15956378
Campylobacter spp. are the second most common pathogen isolated from stools of patients with gastroenteritis in Barbados. The aim of this study was to identify reservoirs of Campylobacter and the likely source(s) of human infection. Fecal specimens from 596 animals and 311 samples of animal food products were analyzed for ... More