角质化细胞 SFM (1X)

角质化细胞 SFM（无血清培养基）经过优化，可用于人角质化细胞和其他类型的上皮细胞的生长和维持，无需细胞饲养层。完全培养基可用于培养人表皮角质化细胞，用于涉及皮肤替代品、体外毒理学和基因治疗的研究。角质化细胞 SFM 是一种无血清完全培养基，在使用时添加了重组人表皮生长因子 (rEGF)了解更多信息

货号	数量
17005042	500 mL

货号 17005042

价格（CNY)

2,168.00

添加至购物车

500 mL

价格（CNY)

2,168.00

添加至购物车

角质化细胞 SFM（无血清培养基）经过优化，可用于人角质化细胞和其他类型的上皮细胞的生长和维持，无需细胞饲养层。完全培养基可用于培养人表皮角质化细胞，用于涉及皮肤替代品、体外毒理学和基因治疗的研究。

角质化细胞 SFM 是一种无血清完全培养基，在使用时添加了重组人表皮生长因子 (rEGF) 和牛脑垂体提取物 (BPE)。两种添加剂 rEGF 和 BPE 与基础培养基分开包装提供。

仅供科研使用。不可用于诊断程序。

细胞类型角质化细胞、上皮细胞

最大浓度1 X

产品类型试剂盒

数量500 mL

有效期每 12 个月

分类无血清

培养类型哺乳动物细胞培养

血清水平无血清

无菌无菌过滤

灭菌方法无菌过滤

加有添加剂谷氨酰胺, 酚红

Unit SizeEach

内容与储存

• 500 mL 角质化细胞-SFM 基础培养基；在冷藏冰箱 (2-8°C) 中避光储存
• 25 mg 牛脑垂体提取物 (BPE)；在冷冻冰箱（-5 至 -20°C）中避光储存
• 2.5 µg 人重组 EGF；在冷冻冰箱（-5 至 -20°C）中避光储存

完全培养基：在冷藏冰箱 (2-8°C) 中避光储存

常见问题解答 (FAQ)

Are the Keratinocyte SFM basal medium and supplement kit available separately?

The basal medium is not available for purchase individually, and can only be ordered as part of the Keratinocyte SFM kit (catalog # 17005-042). The supplements are available separately: Bovine Pituitary Extract is catalog # 13028-014 and the EGF is catalog # PHG0311.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which serum-free media can be used to culture mouse keratinocytes?

The Keratinocyte SFM without calcium chloride (Cat. No. 37010-022) is appropriate for the culture of mouse keratinocytes. The medium should be supplemented with calcium chloride at a final concentration of 0.02 mM.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which serum-free media can be used to culture human keratinocytes?

There are a variety of options for serum-free culture of human keratinocytes. Keratinocyte SFM (Cat. No. 17005-042) is a widely used kit that includes the basal medium, bovine pituitary extract (BPE) and Epidermal Growth Factor (EGF). If you would like a serum-free, defined culture system, the options are the Defined Keratinocyte SFM kit (Cat. No. 10744-019) or Epilife medium (Cat. No. M-EPI-500-CA) supplemented with EDGS (Cat. No. S-012-5). If you would like an animal origin free defined system, Epilife medium (Cat. No. M-EPI-500-CA) with S7 supplement (Cat. No. S-017-5) is an excellent choice.

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What is the calcium concentration in Keratinocyte SFM (1X) (Cat. No. 17005042)?

Keratinocyte SFM (1X) (Cat. No. 17005042) includes Keratinocyte-SFM (Cat. No. 10724011) and Keratinocytes Supplements (Cat. No. 37000015), which contains Bovine Pituitary Extract (BPE) EGF, Human Recombinant. The complete media is prepared by adding the supplements to the base media. The Keratinocyte-SFM (Cat. No. 10724011) contains calcium chloride at 12.98 mg/L and D calcium pantothenate at 0.29 mg/L.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

For how long can I store Keratinocyte-SFM at 4 degrees C?

The base Keratinocyte-SFM has a shelf life of 12 months when stored at 2 to 8 degrees C, protected from light. Keratinocyte-SFM Supplement has a shelf life of 18 months when stored at -20 to 5 degrees C. The complete media should not be stored for longer than 4 weeks at 4 degrees C

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.