Effect of immunohistochemistry on molecular analysis of tissue samples: implications for microdissection technologies.
AuthorsTangrea MA, Mukherjee S, Gao B, Markey SP, Du Q, Armani M, Kreitman MS, Rosenberg AM, Wallis BS, Eberle FC, Duncan FC, Hanson JC, Chuaqui RF, Rodriguez-Canales J, Emmert-Buck MR
JournalJ Histochem Cytochem
PubMed ID21430260
'Laser-based tissue microdissection is an important tool for the molecular evaluation of histological sections. The technology has continued to advance since its initial commercialization in the 1990s, with improvements in many aspects of the process. More recent developments are tailored toward an automated, operator-independent mode that relies on antibodies as ... More
Pleomorphic hyalinizing angiectatic tumor of soft parts: immunohistochemical study including the expression of vascular endothelial growth factor.
AuthorsGroisman GM, Bejar J, Amar M, Ben-Izhak O,
JournalArch Pathol Lab Med
PubMed ID10705399
'We report morphologic, flow cytometric, and immunohistochemical findings in two cases of pleomorphic hyalinizing angiectatic tumor of soft parts. Both patients were middle-aged women with subcutaneous lesions located in the lower extremity. The tumors consisted of sheets of spindled and pleomorphic cells with frequent intranuclear pseudoinclusions associated with clusters of ... More
Bizarre leiomyoma of the scrotum.
AuthorsKim NR, Sung CO, Han J,
JournalJ Korean Med Sci
PubMed ID12808340
Scrotal leiomyomas with atypical bizarre nuclei are rare, which might be misdiagnosed as malignant tumor. We describe a case of scrotal bizarre leiomyoma in a 65-yr-old man. The tumor was a 1 cm-sized, well circumscribed, oval mass arising from the tunica dartos muscle. Histologically, it was formed by whorling bundles ... More
Expression of alpha7beta1 integrin splicing variants during skeletal muscle regeneration.
AuthorsKääriäinen M, Nissinen L, Kaufman S, Sonnenberg A, Järvinen M, Heino J, Kalimo H,
JournalAm J Pathol
PubMed ID12213731
Integrin alpha7beta1 is a laminin receptor, both subunits of which have alternatively spliced, developmentally regulated variants. In skeletal muscle beta1 has two major splice variants of the intracellular domain (beta1A and beta1D). alpha7X1 and alpha7X2 represent variants of the alpha7 ectodomain, whereas alpha7A and alpha7B are variants of the intracellular ... More
Expression of neurofilament proteins in proliferating C2C12 mouse skeletal muscle cells.
AuthorsAbe M, Saitoh O, Nakata H, Yoda A, Matsuda R,
JournalExp Cell Res
PubMed ID8940248
Expression of neurofilament proteins, NF140K, NF68K, and NF200K, in C2C12 mouse skeletal muscle cells was studied. Immunofluorescence and immunoblot analyses revealed that NF140K was expressed in proliferating C2C12 cells and its localization was similar to desmin, a muscle-specific intermediate filament protein. NF140K became undetectable in C2C12 cells as muscle cell ... More
Solitary fibrous tumor of nasal cavity in patient with long-standing history of cocaine inhalation.
AuthorsChauhan SS, Krishnan J, Heffner DK
JournalArch Pathol Lab Med
PubMed ID14692833
We report a solitary fibrous tumor in the nasal cavity of a 48-year-old woman who presented with a history of bilateral nasal obstruction and long-standing cocaine inhalation. Physical examination revealed a large mass involving the right nasal cavity and extending into the posterior aspect of the left nasal cavity. The ... More