'Corin is a multiple-domain type II transmembrane serine protease highly expressed in the heart. It converts pro-atrial natriuretic peptide to atrial natriuretic peptide, a cardiac hormone that regulates blood volume and pressure. Here we describe the genomic structures of the human and murine corin genes and functional analysis of their ... More
Lithium Induces NF-kappa B Activation and Interleukin-8 Production in Human Intestinal Epithelial Cells.
Authors Nemeth Zoltan H; Deitch Edwin A; Szabo Csaba; Fekete Zoltan; Hauser Carl J; Hasko Gyorgy;
JournalJ Biol Chem
PubMed ID11756416
'Lithium has been documented to regulate apoptosis and apoptotic gene expression via NF-kappaB and mitogen-activated protein (MAP) kinase-dependent mechanisms. Since both NF-kappaB and MAP kinases are also important mediators of inflammatory gene expression, we investigated the effect of lithium on NF-kappaB- and MAP kinase-mediated inflammatory gene expression. Incubation of human ... More
Both DNA and histone fold sequences contribute to archaeal nucleosome stability.
Authors Bailey Kathryn A; Marc Frederic; Sandman Kathleen; Reeve John N;
JournalJ Biol Chem
PubMed ID11751933
'The roles and interdependence of DNA sequence and archaeal histone fold structure in determining archaeal nucleosome stability and positioning have been determined and quantitated. The presence of four tandem copies of TTTAAAGCCG in the polylinker region of pLITMUS28 resulted in a DNA molecule with increased affinity (DeltaDeltaG of approximately 700 ... More
Analysis of the hormone-dependent regulation of a JunD-estrogen receptor chimera.
Authors Francis M K; Phinney D G; Ryder K;
JournalJ Biol Chem
PubMed ID7744790
'The modular ligand-binding domains of steroid receptors have been widely used to generate protein chimeras that are ligand dependent for activity. In a similar manner, we generated a series of conditionally active JunD and c-Fos proteins by fusing their carboxyl (COOH)-terminal ends with a COOH-terminal fragment of the human estrogen ... More
Recognition by tryptophanyl-tRNA synthetases of discriminator base on tRNATrp from three biological domains.
Authors Guo Qing; Gong Qingguo; Tong Ka-Lok; Vestergaard Bente; Costa Annie; Desgres Jean; Wong Mansim; Grosjean Henri; Zhu Guang; Wong J Tze-Fei; Xue Hong;
JournalJ Biol Chem
PubMed ID11834741
'To study the recognition by tryptophanyl-tRNA synthetase (TrpRS) of tRNA(Trp) discriminator base, mutations were introduced into the discriminator base of Bacillus subtilis, Archeoglobus fulgidus, and bovine tRNA(Trp), representing the three biological domains. When B. subtilis, A. fulgidus, and human TrpRS were used to acylate these tRNA(Trp), two distinct preference profiles ... More
Transcription and replication initiate at separate sites on the vesicular stomatitis virus genome.
Authors Whelan Sean P J; Wertz Gail W;
JournalProc Natl Acad Sci U S A
PubMed ID12089339
'The RNA-dependent RNA polymerase of the nonsegmented negative-strand RNA viruses carries out two distinct RNA synthetic processes: transcription of monocistronic, capped, and polyadenylated subgenomic messenger RNAs, and replication by means of the synthesis of a full-length positive-sense copy of the genome. The template for both processes is the negative-sense genomic ... More
Modified rapid expansion detection method to analyze Cag/CTG repeat expansions.
Authors Jarjanazi Hamdi; Ozcelik Hilmi;
JournalBiotechniques
PubMed ID12019772
Modified rapid expansion detection method to analyze Cag/CTG repeat expansions.
A simple method using T4 DNA polymerase to clone polymerase chain reaction products.
Authors Wang K; Koop B F; Hood L;
JournalBiotechniques
PubMed ID7980913
None
A structural basis for discriminating between self and nonself double-stranded RNAs in mammalian cells.
AuthorsMarques JT, Devosse T, Wang D, Zamanian-Daryoush M, Serbinowski P, Hartmann R, Fujita T, Behlke MA, Williams BR,
JournalNat Biotechnol
PubMed ID16648842
Nonspecific effects triggered by small interfering RNAs (siRNAs) complicate the use of RNA interference (RNAi) to specifically downregulate gene expression. To uncover the basis of these nonspecific activities, we analyzed the effect of chemically synthesized siRNAs on mammalian double-stranded RNA (dsRNA)-activated signaling pathways. siRNAs ranging from 21 to 27 nucleotides ... More
Principles of 3' splice site selection and alternative splicing for an unusual group II intron from Bacillus anthracis.
AuthorsRobart AR, Montgomery NK, Smith KL, Zimmerly S,
JournalRNA
PubMed ID15100440
We investigated the self-splicing properties of two introns from the bacterium Bacillus anthracis. One intron (B.a.I1) splices poorly in vitro despite having typical structural motifs, while the second (B.a.I2) splices well while having apparently degenerated features. The spliced exons of B.a.I2 were sequenced, and splicing was found to occur at ... More
Structural determinants for HIV-1 integrase inhibition by beta-diketo acids.
Authors Marchand Christophe; Zhang Xuechun; Pais Godwin C G; Cowansage Kiriana; Neamati Nouri; Burke Terrence R Jr; Pommier Yves;
JournalJ Biol Chem
PubMed ID11805103
Among all the HIV-1 integrase inhibitors, the beta-diketo acids (DKAs) represent a major lead in anti-HIV-1 integrase drug design. These derivatives inhibit the integration reaction in vitro with a strong specificity for the 3'-end joining step. They are also antiviral and inhibit integration in vivo. The aim of the present ... More
DNA repair of a single UV photoproduct in a designed nucleosome.
Authors Kosmoski J V; Ackerman E J; Smerdon M J;
JournalProc Natl Acad Sci U S A
PubMed ID11517308
Eukaryotic DNA repair enzymes must interact with the architectural hierarchy of chromatin. The challenge of finding damaged DNA complexed with histone proteins in nucleosomes is complicated by the need to maintain local chromatin structures involved in regulating other DNA processing events. The heterogeneity of lesions induced by DNA-damaging agents has ... More
The RecA intein of Mycobacterium tuberculosis promotes cleavage of ectopic DNA sites. Implications for the dispersal of inteins in natural populations.
Authors Guhan N; Muniyappa K;
JournalJ Biol Chem
PubMed ID12167644
The RecA intein of Mycobacterium tuberculosis, a novel double-stranded DNA endonuclease, requires both Mn(2+) and ATP for efficient cleavage of the inteinless recA allele. In this study, we show that Mg(2+) alone was sufficient to stimulate PI-MtuI to cleave double-stranded DNA at ectopic sites. In the absence of Mg(2+), PI-MtuI ... More
Cell-cycle-dependent Regulation of Human aurora A Transcription Is Mediated by Periodic Repression of E4TF1.
Human aurora A is a serine-threonine kinase that controls various mitotic events. The transcription of aurora A mRNA varies throughout the cell cycle and peaks during G(2)/M. To clarify the transcriptional mechanism, we first cloned the 1.8-kb 5'-flanking region of aurora A including the first exon. Transient expression of aurora ... More
Mycobacterium tuberculosis RecA Intein Possesses a Novel ATP-dependent Site-specific Double-stranded DNA Endonuclease Activity.
Authors Guhan N; Muniyappa K;
JournalJ Biol Chem
PubMed ID11850426
Mycobacterium tuberculosis recA harbors an intervening sequence in its open reading frame, presumed to encode an endonuclease (PI-MtuI) required for intein homing in inteinless recA allele. Although the protein-splicing ability of PI-MtuI has been characterized, the identification of its putative endonuclease activity has remained elusive. To investigate whether PI-MtuI possesses ... More
Identification of sterol-independent regulatory elements in the human ATP-binding cassette transporter A1 promoter: role of Sp1/3, E-box binding factors, and an oncostatin M-responsive element.
The ATP-binding cassette transporter A1 (ABCA1) shows a differentiation-, cAMP-, and sterol-dependent up-regulation in human monocytes. As part of an ongoing study, we investigated the proximal promoter regions that are highly conserved between the human and murine ABCA1 genes. Using reporter gene assays, we show here that a TATA box ... More