Analysis of the hormone-dependent regulation of a JunD-estrogen receptor chimera.
Authors Francis M K; Phinney D G; Ryder K;
JournalJ Biol Chem
PubMed ID7744790
'The modular ligand-binding domains of steroid receptors have been widely used to generate protein chimeras that are ligand dependent for activity. In a similar manner, we generated a series of conditionally active JunD and c-Fos proteins by fusing their carboxyl (COOH)-terminal ends with a COOH-terminal fragment of the human estrogen ... More
Modified rapid expansion detection method to analyze Cag/CTG repeat expansions.
Authors Jarjanazi Hamdi; Ozcelik Hilmi;
JournalBiotechniques
PubMed ID12019772
Modified rapid expansion detection method to analyze Cag/CTG repeat expansions.
Principles of 3' splice site selection and alternative splicing for an unusual group II intron from Bacillus anthracis.
AuthorsRobart AR, Montgomery NK, Smith KL, Zimmerly S,
JournalRNA
PubMed ID15100440
We investigated the self-splicing properties of two introns from the bacterium Bacillus anthracis. One intron (B.a.I1) splices poorly in vitro despite having typical structural motifs, while the second (B.a.I2) splices well while having apparently degenerated features. The spliced exons of B.a.I2 were sequenced, and splicing was found to occur at ... More