Role of the E1A Rb-binding domain in repression of the NF-kappa B-dependent defense against tumor necrosis factor-alpha.
Authors Cook James L; Walker Thomas A; Worthen G Scott; Radke Jay R;
JournalProc Natl Acad Sci U S A
PubMed ID12119420
'The adenoviral E1A oncogene sensitizes mammalian cells to tumor necrosis factor-alpha (TNF-alpha), in part by repressing the nuclear factor-kappa B (NF-kappa B)-dependent defense against this cytokine. Other E1A activities involve binding to either p300/cyclic AMP response element-binding protein (CBP) or retinoblastoma (Rb)-family proteins, but the roles of E1A interactions with ... More
Identification of a Novel Transcription Factor, GAGATA-binding Protein, Involved in Androgen-mediated Expression of Prostate-specific Antigen.
AuthorsWang C, Yeung F, Liu PC, Attar RM, Geng J, Chung LW, Gottardis M, Kao C,
JournalJ Biol Chem
PubMed ID12782640
'Prostate-specific antigen (PSA) is the most valuable marker for the evaluation of prostate cancer progression. The expression of PSA is controlled by androgen receptor (AR) through its binding to androgen-response elements (AREs). Several AREs have been identified within the 5.8-kb PSA promoter. The main activity of this 5.8-kb PSA promoter ... More
Characterization of the SECIS binding protein 2 complex required for the co-translational insertion of selenocysteine in mammals.
AuthorsKinzy SA, Caban K, Copeland PR,
JournalNucleic Acids Res
PubMed ID16155186
'Selenocysteine is incorporated into at least 25 human proteins by a complex mechanism that is a unique modification of canonical translation elongation. Selenocysteine incorporation requires the concerted action of a kink-turn structural RNA (SECIS) element in the 3'' untranslated region of each selenoprotein mRNA, a selenocysteine-specific translation elongation factor (eEFSec) ... More
A simple method using T4 DNA polymerase to clone polymerase chain reaction products.