'Chronic variable stress (CVS) exposure modifies the paraventricular nucleus of the hypothalamus (PVN) in a manner consistent with enhanced central drive of the hypothalamo-pituitary-adrenocortical (HPA) axis. As previous reports suggest that post-stress enhancement of norepinephrine (NE) action contributes to chronic stress regulation at the level of the PVN, we hypothesised ... More
Light microscopic identification and immunocytochemical characterization of glutamatergic synapses in brain sections.
AuthorsMelone M, Burette A, Weinberg RJ
JournalJ Comp Neurol
PubMed ID16228991
'Presynaptic proteins are readily identified by light microscopic immunocytochemistry, but immunodetection of postsynaptic proteins in brain sections proves difficult. We performed immunofluorescent double labeling for the NR1 subunit of the N-methyl-D-aspartate receptor (NMDAR) and the vesicular glutamate transporter 1 (VGLUT1). In material fixed with 4% paraformaldehyde, NMDAR staining in somatosensory ... More
Combined pleomorphic xanthoastrocytoma-ganglioglioma of the cerebellum.
AuthorsEvans AJ, Fayaz I, Cusimano MD, Laperriere N, Bilbao JM,
JournalArch Pathol Lab Med
PubMed ID11079032
'Combined pleomorphic xanthoastrocytoma-ganglioma is a rare neoplasm, occurring in patients younger than 30 years. The clinical course of these tumors is difficult to predict because of their rarity. We report a case of combined pleomorphic xanthoastrocytoma-ganglioma that, in addition to the patient''s age, is unusual in several respects. The lesion ... More
Critical function for ADAM9 in mouse prostate cancer.
AuthorsPeduto L, Reuter VE, Shaffer DR, Scher HI, Blobel CP,
JournalCancer Res
PubMed ID16230393
'ADAM9 is a membrane-anchored metalloprotease that is markedly up-regulated in several human carcinomas. Here, we show that ADAM9 is similarly up-regulated in mouse models for prostate, breast, and intestinal carcinoma. To assess whether ADAM9 is critical for the pathogenesis of prostate carcinoma, one of the most common cancers in men, ... More
Corticomedullary mixed tumor of the adrenal gland with concurrent adrenal myelolipoma.
AuthorsChu AY, LiVolsi VA, Fraker DL, Zhang PJ
JournalArch Pathol Lab Med
PubMed ID12873195
We report the case of a corticomedullary mixed tumor of the adrenal gland in a 55-year-old woman with a left adrenal mass who presented with mild symptoms of Cushing syndrome and an elevated urinary cortisol level. The patient underwent a left adrenalectomy. A well-circumscribed 2.5-cm mass, composed of an admixture ... More
A neuronal isoform of CPEB regulates local protein synthesis and stabilizes synapse-specific long-term facilitation in aplysia.
AuthorsSi K, Giustetto M, Etkin A, Hsu R, Janisiewicz AM, Miniaci MC, Kim JH, Zhu H, Kandel ER,
JournalCell
PubMed ID14697206
Synapse-specific facilitation requires rapamycin-dependent local protein synthesis at the activated synapse. In Aplysia, rapamycin-dependent local protein synthesis serves two functions: (1) it provides a component of the mark at the activated synapse and thereby confers synapse specificity and (2) it stabilizes the synaptic growth associated with long-term facilitation. Here we ... More
Primary malignant melanoma of the common bile duct: a case report and review of the literature.
AuthorsWagner MS, Shoup M, Pickleman J, Yong S
JournalArch Pathol Lab Med
PubMed ID10705398
Primary malignant melanoma of the common bile duct is rare. To our knowledge, only 6 cases have been reported previously. The pathologic diagnosis of primary malignant melanoma in extracutaneous sites often requires the use of confirmatory immunohistochemical stains and electron microscopy studies, as well as tests to rule out other ... More
Trafficking of GABA(A) receptors, loss of inhibition, and a mechanism for pharmacoresistance in status epilepticus.
AuthorsNaylor DE, Liu H, Wasterlain CG,
JournalJ Neurosci
PubMed ID16120773
During status epilepticus (SE), GABAergic mechanisms fail and seizures become self-sustaining and pharmacoresistant. During lithiumpilocarpine-induced SE, our studies of postsynaptic GABA(A) receptors in dentate gyrus granule cells show a reduction in the amplitude of miniature IPSCs (mIPSCs). Anatomical studies show a reduction in the colocalization of the beta2/beta3 and gamma2 ... More
Neurotransmitter release regulated by a MALS-liprin-alpha presynaptic complex.
AuthorsOlsen O, Moore KA, Fukata M, Kazuta T, Trinidad JC, Kauer FW, Streuli M, Misawa H, Burlingame AL, Nicoll RA, Bredt DS,
JournalJ Cell Biol
PubMed ID16186258
Synapses are highly specialized intercellular junctions organized by adhesive and scaffolding molecules that align presynaptic vesicular release with postsynaptic neurotransmitter receptors. The MALS/Veli-CASK-Mint-1 complex of PDZ proteins occurs on both sides of the synapse and has the potential to link transsynaptic adhesion molecules to the cytoskeleton. In this study, we ... More
Role of gap junctions in fluid secretion of lacrimal glands.
AuthorsWalcott B, Moore LC, Birzgalis A, Claros N, Valiunas V, Ott T, Willecke K, Brink PR,
JournalAm J Physiol Cell Physiol
PubMed ID11832335
In glands such as the liver and pancreas, gap junctions containing connexin 26 and 32 (Cx26 and Cx32, respectively) couple the secretory cells. Uncoupling these junctions compromises the secretory function of these glands. Lacrimal glands also contain extensive arrays of gap junctions consisting of Cx26 and Cx32. We wanted to ... More
Impaired motoneuronal retrograde transport in two models of SBMA implicates two sites of androgen action.
AuthorsKemp MQ, Poort JL, Baqri RM, Lieberman AP, Breedlove SM, Miller KE, Jordan CL
JournalHum Mol Genet
PubMed ID21873607
Spinal and bulbar muscular atrophy (SBMA) impairs motor function in men and is linked to a CAG repeat mutation in the androgen receptor (AR) gene. Defects in motoneuronal retrograde axonal transport may critically mediate motor dysfunction in SBMA, but the site(s) where AR disrupts transport is unknown. We find deficits ... More
Muscle-derived extracellular signal-regulated kinases 1 and 2 are required for the maintenance of adult myofibers and their neuromuscular junctions.
AuthorsSeaberg B, Henslee G, Wang S, Paez-Colasante X, Landreth GE, Rimer M
Journal
PubMed ID25605336
The Ras-extracellular signal-regulated kinase 1 and 2 (ERK1/2) pathway appears to be important for the development, maintenance, aging, and pathology of mammalian skeletal muscle. Yet no gene targeting of Erk1/2 in muscle fibers in vivo has been reported to date. We combined a germ line Erk1 mutation with Cre-loxP Erk2 ... More
Combinatorial homophilic interaction between gamma-protocadherin multimers greatly expands the molecular diversity of cell adhesion.
AuthorsSchreiner D, Weiner JA
JournalProc Natl Acad Sci U S A
PubMed ID20679223
The specificity of interactions between neurons is believed to be mediated by diverse cell adhesion molecules, including members of the cadherin superfamily. Whereas mechanisms of classical cadherin adhesion have been studied extensively, much less is known about the related protocadherins (Pcdhs), which together make up the majority of the superfamily. ... More
Growth differentiation factor 6 as a putative risk factor in neuromuscular degeneration.
Mutation of Glass bottom boat, the Drosophila homologue of the bone morphogenetic protein or growth/differentiation factor (BMP/GDF) family of genes in vertebrates, has been shown to disrupt development of neuromuscular junctions (NMJ). Here we tested whether this same conclusion can be broadened to vertebrate BMP/GDF genes. This analysis was also ... More
Developmental-dependent action of microtubule depolymerization on the function and structure of synaptic glycine receptor clusters in spinal neurons.
Authorsvan Zundert B, Alvarez FJ, Tapia JC, Yeh HH, Diaz E, Aguayo LG
JournalJ Neurophysiol
PubMed ID12968009
Microtubules have been proposed to interact with gephyrin/glycine receptors (GlyRs) in synaptic aggregates. However, the consequence of microtubule disruption on the structure of postsynaptic GlyR/gephyrin clusters is controversial and possible alterations in function are largely unknown. In this study, we have examined the physiological and morphological properties of GlyR/gephyrin clusters ... More