Effective application of the methanol-based PreservCyt(™) fixative and the Cellient(™) automated cell block processor to diagnostic cytopathology, immunocytochemistry, and molecular biology.
Authorsvan Hemel BM, Suurmeijer AJ,
JournalDiagn Cytopathol
PubMed ID23444168
'We studied the feasibility of immunocytochemistry (ICC), in situ hybridization (ISH), and polymerase chain reaction (PCR) after Cellient(™) automated cell block processing, and tested whether methanol-based PreservCyt(™) fixation could replace formalin fixation, in an attempt to eliminate toxic formaldehyde vapors. Immunostaining with 30 different antibodies was performed on cell blocks ... More
Is mammary not otherwise specified-type sarcoma with CD10 expression a distinct entity? A rare case report with immunohistochemical and ultrastructural study.
AuthorsYang GZ, Li J, Jin H, Ding HY,
JournalDiagn Pathol
PubMed ID23356903
'Mammary sarcoma is extremely rare and the diagnosis is established only after metaplastic carcinomas and malignant phyllodes tumours are excluded. A rare case of not otherwise specified-type sarcoma with CD10 expression in the left breast in a 45-year-old female was presented. It was a high-grade tumour composed of spindle cells ... More
Florid decidual reaction mimicking gastrointestinal malignancy in a primipara woman.
AuthorsRodriguez FJ, Abraham SC, Sendelbach KM, Nascimento AG
JournalHistopathology
PubMed ID16842249
Molecular-based tumour subtypes of canine mammary carcinomas assessed by immunohistochemistry.
AuthorsSassi F, Benazzi C, Castellani G, Sarli G,
JournalBMC Vet Res
PubMed ID20109214
Human breast cancer is classified by gene expression profile into subtypes consisting of two hormone (oestrogen and/or progesterone) receptor-positive types (luminal-like A and luminal-like B) and three hormone receptor-negative types [human epidermal growth factor receptor 2-expressing, basal-like, and unclassified ( ... More
Subtyping of breast cancer by immunohistochemistry to investigate a relationship between subtype and short and long term survival: a collaborative analysis of data for 10,159 cases from 12 studies.
AuthorsBlows FM, Driver KE, Schmidt MK, Broeks A, van Leeuwen FE, Wesseling J, Cheang MC, Gelmon K, Nielsen TO, Blomqvist C, Heikkilä P, Heikkinen T, Nevanlinna H, Akslen LA, Bégin LR, Foulkes WD, Couch FJ, Wang X, Cafourek V, Olson JE, Baglietto L, Giles GG, Severi G, McLean CA, Southey MC, Rakha E, Green AR, Ellis IO, Sherman ME, Lissowska J, Anderson WF, Cox A, Cross SS, Reed MW, Provenzano E, Dawson SJ, Dunning AM, Humphreys M, Easton DF, García-Closas M, Caldas C, Pharoah PD, Huntsman D
JournalPLoS Med
PubMed ID20520800
Immunohistochemical markers are often used to classify breast cancer into subtypes that are biologically distinct and behave differently. The aim of this study was to estimate mortality for patients with the major subtypes of breast cancer as classified using five immunohistochemical markers, to investigate patterns of mortality over time, and ... More
Expression of Lewis X is associated with poor prognosis in triple-negative breast cancer.
AuthorsKoh YW, Lee HJ, Ahn JH, Lee JW, Gong G,
Journal
PubMed ID23690116
The Lewis X (Le(X)) antigen is a prognostic marker in certain solid tumors and has been proposed as a therapeutic target. Immunohistochemical analyses were performed to retrospectively examine the prognostic impact of Le(X) expression in 158 patients with triple-negative breast cancer (TNBC). Twenty-three (14.5%) patients were Le(X)-positive. Multivariate analysis showed ... More
An associated classification of triple negative breast cancer: the risk of relapse and the response to chemotherapy.
AuthorsZhang J, Wang Y, Yin Q, Zhang W, Zhang T, Niu Y,
Journal
PubMed ID23826420
Triple negative breast cancer (TNBC) is heterogeneous and considered as an aggressive tumor. This study was to evaluate the associated classification and its correlations with prognosis and the response to chemotherapy in Chinese women. Four hundred and twenty-eight cases of invasive TNBC were involved in this study. The expression of ... More