Isolation and characterization of the human cytochrome P450 CYP1B1 gene.
Authors Tang Y M; Wo Y Y; Stewart J; Hawkins A L; Griffin C A; Sutter T R; Greenlee W F;
JournalJ Biol Chem
PubMed ID8910454
'Previously, we identified a novel human cytochrome P450 cDNA that is inducible by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and represents the first member of a new subfamily designated cytochrome P4501B1 (CYP1B1; Sutter, T. R., Tang, Y. M., Hayes, C. L., Wo, Y. P., Jabs, E. W., Li, X., Yin, H., Cody, C. W., ... More
An imprinted PEG1/MEST antisense expressed predominantly in human testis and in mature spermatozoa.
Authors Li Tao; Vu Thanh H; Lee Kok-Onn; Yang Youwen; Nguyen Chuyen V; Bui Huy Q; Zeng Zhi-Lan; Nguyen Binh T; Hu Ji-Fan; Murphy Susan K; Jirtle Randy L; Hoffman Andrew R;
JournalJ Biol Chem
PubMed ID11821432
'PEG1 (or MEST) is an imprinted gene located on human chromosome 7q32 that is expressed predominantly from the paternal allele. In the mouse, Peg1/Mest is associated with embryonic growth and maternal behavior. Human PEG1 is transcribed from two promoters; the transcript from promoter P1 is derived from both parental alleles, ... More
Global Expression Profiling of Acetate-grown Escherichia coli.
Authors Oh Min-Kyu; Rohlin Lars; Kao Katy C; Liao James C;
JournalJ Biol Chem
PubMed ID11815613
'This study characterized the transcript profile of Escherichia coli in acetate cultures using DNA microarray on glass slides. Glucose-grown cultures were used as a reference. At the 95% confidence level, 354 genes were up-regulated in acetate, while 370 genes were down-regulated compared with the glucose-grown culture. Generally, more metabolic genes ... More
Tumor necrosis factor alpha increases the expression of glycosyltransferases and sulfotransferases responsible for the biosynthesis of sialylated and/or sulfated Lewis x epitopes in the human bronchial mucosa.
'There is increasing evidence that inflammation may affect glycosylation and sulfation of various glycoproteins. The present study reports the effect of tumor necrosis factor alpha (TNF-alpha), a proinflammatory cytokine, on the glycosyl- and sulfotransferases of the human bronchial mucosa responsible for the biosynthesis of Lewis x epitope and of its ... More
Methylation-mediated silencing of TMS1/ASC is accompanied by histone hypoacetylation and CpG island-localized changes in chromatin architecture.
Authors Stimson Krista M; Vertino Paula M;
JournalJ Biol Chem
PubMed ID11733524
'Aberrant methylation of CpG-dense islands in the promoter regions of genes is an acquired epigenetic alteration associated with the silencing of tumor suppressor genes in human cancers. In a screen for endogenous targets of methylation-mediated gene silencing, we identified a novel CpG island-associated gene, TMS1, which is aberrantly methylated and ... More
Dome formation in cell cultures as expression of an early stage of lactogenic differentiation of the mammary gland.
'The study of the development of the mammary gland at the molecular level in the animal is difficult because of the complex tissue organization of the gland. We have previously developed an in vitro system for genetic analysis of mammary cell differentiation, based on the cell line LA7 clonally derived ... More
The role of endogenous human Trp4 in regulating carbachol-induced calcium oscillations in HEK-293 cells.
'We utilized 2-aminoethyoxydiphenyl borane, an agent that blocks store-operated Ca(2+) entry, as well as an antisense approach to characterize endogenous Ca(2+) entry pathways in HEK-293 cells. The thapsigargin- and carbachol-induced, but not the 1-oleolyl-2-acytyl-sn-glycerol (OAG)-induced, entry was blocked by 2-aminoethyoxydiphenyl borane. Both reverse transcriptase-PCR and Western blot analyses demonstrated endogenous ... More
Regulation of stress response signaling by the N-terminal dishevelled/EGL-10/pleckstrin domain of Sst2, a regulator of G protein signaling in Saccharomyces cerevisiae.
Authors Burchett Scott A; Flanary Paul; Aston Christopher; Jiang Lixin; Young Kathleen H; Uetz Peter; Fields Stanley; Dohlman Henrik G;
JournalJ Biol Chem
PubMed ID11940600
'All members of the regulator of G protein signaling (RGS) family contain a conserved core domain that can accelerate G protein GTPase activity. The RGS in yeast, Sst2, can inhibit a G protein signal leading to mating. In addition, some RGS proteins contain an N-terminal domain of unknown function. Here ... More
Functional importance of Stat3 tetramerization in activation of the alpha 2-macroglobulin gene.
Authors Zhang X; Darnell J E Jr;
JournalJ Biol Chem
PubMed ID11438543
'A tetrameric Stat3 complex was found to be essential in transfection experiments for maximal interleukin-6-inducible activation of alpha2-macroglobulin gene promoter. Stable tetramer formation of purified phosphorylated Stat3 was dependent on protein.protein interaction involving the N-terminal domain of Stat3. The functional importance of tetramer formation was shown by the decreased levels ... More
Molecular basis defining human Chlamydia trachomatis tissue tropism. A possible role for tryptophan synthase.
Authors Fehlner-Gardiner Christine; Roshick Christine; Carlson John H; Hughes Scott; Belland Robert J; Caldwell Harlan D; McClarty Grant;
JournalJ Biol Chem
PubMed ID12011099
Here we report the cloning and sequencing of a region of the chlamydiae chromosome termed the
A Requirement for the CD44 Cytoplasmic Domain for Hyaluronan Binding, Pericellular Matrix Assembly, and Receptor-mediated Endocytosis in COS-7 Cells.
Authors Jiang Hong; Peterson Richard S; Wang Weihua; Bartnik Eckart; Knudson Cheryl B; Knudson Warren;
JournalJ Biol Chem
PubMed ID11792695
CD44-negative COS-7 cells were transfected with expression constructs for CD44H (the predominant CD44 isoform), CD44E (epithelial isoform), or truncation mutant derivatives lacking the carboxyl-terminal 67 amino acids of the cytoplasmic domain, CD44HDelta67 and CD44EDelta67. The truncation mutant CD44HDelta67 is identical to a naturally occurring alternatively spliced ... More
Mitogen-activated protein kinase signaling in postgermination arrest of development by abscisic acid.
Authors Lu C; Han M-H; Guevara-Garcia A; Fedoroff N V;
JournalProc Natl Acad Sci U S A
PubMed ID12434021
Abscisic acid (ABA) mediates plant responses to environmental stress, particularly to water status. During germination, the embryo emerges from dormancy as the ABA concentration declines. Exposure to exogenous ABA during germination arrests development rapidly, but reversibly, enabling seedlings to withstand early water stress without loss of viability. Postgermination proteolytic degradation ... More
Suppressor of Cytokine Signaling 3 Regulates Proliferation and Activation of T-helper Cells.
Suppressors of cytokine signaling (SOCS) have been implicated in regulation of T-cell activation and cytokine-mediated differentiation of T-helper cells. In this study we have characterized the pattern of SOCS expression in naïve and activated primary T-helper cells, examined whether expression of SOCS genes is regulated by cytokine or T-cell receptor ... More
Infectivity-associated changes in the transcriptional repertoire of the malaria parasite sporozoite stage.
Authors Matuschewski Kai; Ross Jessica; Brown Stuart M; Kaiser Karine; Nussenzweig Victor; Kappe Stefan H I;
JournalJ Biol Chem
PubMed ID12177071
Injection of Plasmodium salivary gland sporozoites into the vertebrate host by Anopheles mosquitoes initiates malaria infection. Sporozoites develop within oocysts in the mosquito midgut and then enter and mature in the salivary glands. Although morphologically similar, oocyst sporozoites and salivary gland sporozoites differ strikingly in their infectivity to the mammalian ... More
Monoallelic expression and methylation of imprinted genes in human and mouse embryonic germ cell lineages.
Authors Onyango Patrick; Jiang Shan; Uejima Hiroshi; Shamblott Michael J; Gearhart John D; Cui Hengmi; Feinberg Andrew P;
JournalProc Natl Acad Sci U S A
PubMed ID12149520
Imprinting is an epigenetic modification leading to monoallelic expression of some genes, and disrupted imprinting is believed to be a barrier to human stem cell transplantation, based on studies that suggest that epigenetic marks are unstable in mouse embryonic germ (EG) and embryonic stem (ES) cells. However, stem cell imprinting ... More
A novel S-adenosyl-L-methionine:arsenic(III) methyltransferase from rat liver cytosol.
Authors Lin Shan; Shi Qing; Nix F Brent; Styblo Miroslav; Beck Melinda A; Herbin-Davis Karen M; Hall Larry L; Simeonsson Josef B; Thomas David J;
JournalJ Biol Chem
PubMed ID11790780
S-Adenosyl-l-methionine (AdoMet):arsenic(III) methyltransferase, purified from liver cytosol of adult male Fischer 344 rats, catalyzes transfer of a methyl group from AdoMet to trivalent arsenicals producing methylated and dimethylated arsenicals. The kinetics of production of methylated arsenicals in reaction mixtures containing enzyme, AdoMet, dithiothreitol, glutathione (GSH), and arsenite are consistent with ... More